PMID- 17954268
OWN - NLM
STAT- MEDLINE
DCOM- 20071206
LR  - 20120604
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 178
IP  - 2
DP  - 2007 Oct 15
TI  - A break-apart fluorescence in situ hybridization assay for detecting RET 
      translocations in papillary thyroid carcinoma.
PG  - 128-34
AB  - At least 15 different translocations have been described activating RET in 
      papillary thyroid carcinomas. A break-apart fluorescence in situ hybridization 
      (FISH) assay should detect many translocations involving the RET gene without 
      requiring knowledge of the partner gene. G-banding and spectral karyotyping was 
      performed to further characterize the papillary carcinoma cell line TPC-1. BAC 
      clones flanking the 5' and 3' regions of RET were labeled with SpectrumRed and 
      biotin detected with avidin-AMCA (blue). In addition to the previously described 
      chromosomal t(1;10;21), TPC-1 was found to have del(7)(q22q31) and 
      der(8)t(8;8)(p21;q11.2). With the BAC probes, TPC-1 interphase nuclei showed the 
      expected signal pattern of one paired red-blue signal as well as separated red 
      and blue signals from the rearranged RET gene in 93% of cells. Interphase nuclei 
      from normal human lymphocytes showed two paired red-blue signals in 97% of cells. 
      TPC-1 cells were found to have the previously described chromosomal rearrangement 
      that involves chromosome 10, with few other cytogenetically detectable genomic 
      alterations. RET rearrangement can be detected by a break-apart BAC FISH probe 
      set in the interphase nuclei of TPC-1 cells. This assay can potentially detect 
      clinically relevant translocations involving RET.
FAU - Chen, Frank
AU  - Chen F
AD  - Department of Pathology, The Johns Hopkins Hospital, Baltimore, MD 21287, USA.
FAU - Clark, Douglas P
AU  - Clark DP
FAU - Hawkins, Anita L
AU  - Hawkins AL
FAU - Morsberger, Laura A
AU  - Morsberger LA
FAU - Griffin, Constance A
AU  - Griffin CA
LA  - eng
GR  - T32 CA67751/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-ret)
RN  - EC 2.7.10.1 (RET protein, human)
SB  - IM
MH  - Carcinoma, Papillary/*genetics
MH  - Cell Line, Tumor
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 10
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Proto-Oncogene Proteins c-ret/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Thyroid Neoplasms/*genetics
MH  - Translocation, Genetic/*genetics
EDAT- 2007/10/24 09:00
MHDA- 2007/12/07 09:00
CRDT- 2007/10/24 09:00
PHST- 2007/07/12 00:00 [received]
PHST- 2007/07/13 00:00 [accepted]
PHST- 2007/10/24 09:00 [pubmed]
PHST- 2007/12/07 09:00 [medline]
PHST- 2007/10/24 09:00 [entrez]
AID - S0165-4608(07)00433-5 [pii]
AID - 10.1016/j.cancergencyto.2007.07.006 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2007 Oct 15;178(2):128-34. doi: 
      10.1016/j.cancergencyto.2007.07.006.