PMID- 17955291
OWN - NLM
STAT- MEDLINE
DCOM- 20081104
LR  - 20211020
IS  - 1436-2228 (Print)
IS  - 1436-2228 (Linking)
VI  - 10
IP  - 2
DP  - 2008 Mar-Apr
TI  - FISH mapping and identification of Zhikong scallop (Chlamys farreri) chromosomes.
PG  - 151-7
AB  - Chromosome identification is the first step in genomic research of a species, but 
      it remains a challenge in scallops. In the present study, fluorescence in situ 
      hybridization (FISH) mapping of 19 fosmid clones was attempted and used for 
      chromosome identification in Zhikong scallop (Chlamys farreri Jones et Preston, 
      1904). Data showed that 10 clones were successfully mapped, including 7 without 
      and 3 with C ( 0 ) t-1 DNA. Among them, 2 represented multiple signals and made 
      no contribution to chromosome identification. Karyotypic analysis and 
      cohybridization indicated that the remaining 8 clones realized the identification 
      of 8 chromosomes. All 10 clones were sequenced at both ends, which could be 
      developed as sequence-tagged sites and used for the unification of the 
      cytological and genetic linkage maps. This study shows that fosmid clones can 
      benefit chromosome identification and will undoubtedly be useful for cytogenetic 
      research in Zhikong scallop.
FAU - Zhang, Lingling
AU  - Zhang L
AD  - Division of Life Science and Technology, Ocean University of China, Qingdao, 
      266003, People's Republic of China.
FAU - Bao, Zhenmin
AU  - Bao Z
FAU - Wang, Shi
AU  - Wang S
FAU - Hu, Xiaoli
AU  - Hu X
FAU - Hu, Jingjie
AU  - Hu J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071023
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
SB  - IM
MH  - Animals
MH  - Chromosome Mapping/*methods
MH  - Chromosomes/*genetics/metabolism
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Karyotyping/veterinary
MH  - Metaphase
MH  - Pectinidae/*genetics
EDAT- 2007/10/24 09:00
MHDA- 2008/11/05 09:00
CRDT- 2007/10/24 09:00
PHST- 2007/03/25 00:00 [received]
PHST- 2007/07/10 00:00 [accepted]
PHST- 2007/07/07 00:00 [revised]
PHST- 2007/10/24 09:00 [pubmed]
PHST- 2008/11/05 09:00 [medline]
PHST- 2007/10/24 09:00 [entrez]
AID - 10.1007/s10126-007-9045-x [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2008 Mar-Apr;10(2):151-7. doi: 10.1007/s10126-007-9045-x. 
      Epub 2007 Oct 23.