PMID- 17956026
OWN - NLM
STAT- MEDLINE
DCOM- 20071220
LR  - 20181201
IS  - 0300-0729 (Print)
IS  - 0300-0729 (Linking)
VI  - 45
IP  - 3
DP  - 2007 Sep
TI  - IL-4 and TNF-alpha increased the secretion of eotaxin from cultured fibroblasts 
      of nasal polyps with eosinophil infiltration.
PG  - 235-41
AB  - BACKGROUND: Nasal polyposis is considered a subgroup of chronic rhinosinusitis 
      (CRS). Eosinophils are the most common inflammatory cells in nasal polyp and the 
      degree of the tissue eosinophilia is correlated with the probability of the 
      recurrence of nasal polyps. However, the mechanism by which eosinophils are 
      selectively recruited in nasal polyp remains to be clarified. In the present 
      study, fibroblasts were isolated from nasal polyps of patients with 
      eosinophil-rich nasal polyps (Enp) and those with non-eosinophilic nasal polyps 
      (NEnp) and the secreted levels of eotaxin, regulated upon activation normal T 
      expressed and presumably secreted (RANTES), and vascular cell adhesion molecule-1 
      (VCAM-1) from the cultured fibroblasts were determined. The levels were compared 
      between Enp and Nenp. The role of those chemokines and adhesion molecules in the 
      pathogenesis of nasal polyp is discussed. METHODS: Fibroblasts isolated from 
      nasal polyps of five patients with CRS with Enp and four patients with CRS with 
      NEnp were cultured and stimulated with 10 ng/ml of tumor necrosis factor-alpha 
      (TNF-alpha) and interleukin-4 (IL-4) for 24 hours. After stimulation, culture 
      supernatants were collected and concentrations of eotaxin, RANTES, and VCAM-1 
      were quantified by Enzyme linked immunosorbent assay (ELISA). RESULTS: TNF-alpha 
      enhanced the secretion of VCAM-1 and RANTES by fibroblasts derived from both NEnp 
      and Enp, but did not affect the release of eotaxin. IL-4 increased the secretion 
      of VCAM-1 and eotaxin but not that of RANTES. Furthermore, TNF-alpha and IL-4, 
      when added together, induced a synergistic effect on the secretion of VCAM-1 and 
      eotaxin. The effect of IL-4 and IL-4 plus TNF-alpha on eotaxin release was more 
      marked for Enp fibroblasts compared with NEnp fibroblasts. CONCLUSIONS: The 
      results suggest that eotaxin plays an important role in the selective recruitment 
      of eosinophils in Enp. Nasal fibroblasts in Enp are more sensitive than those in 
      NEnp regarding eotaxin release induced by the stimulation with IL-4 and 
      co-stimulation with TNF-alpha and IL-4. This difference might be associated with 
      the pathogenesis of nasal polyposis having marked accumulation of eosinophils.
FAU - Yoshifuku, Kousuke
AU  - Yoshifuku K
AD  - Department of Otolaryngology Head and Neck Surgery, Kagoshima University Graduate 
      School of Medical and Dental Sciences, Kagoshima, Japan.
FAU - Matsune, Shoji
AU  - Matsune S
FAU - Ohori, Junichiro
AU  - Ohori J
FAU - Sagara, Yukari
AU  - Sagara Y
FAU - Fukuiwa, Tatsuya
AU  - Fukuiwa T
FAU - Kurono, Yuichi
AU  - Kurono Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Rhinology
JT  - Rhinology
JID - 0347242
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines, CC)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Cells, Cultured
MH  - Chemokine CCL5
MH  - Chemokines, CC/*metabolism
MH  - Eosinophils/*metabolism
MH  - Female
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Interleukin-4/*physiology
MH  - Male
MH  - Middle Aged
MH  - Nasal Polyps/*metabolism
MH  - Tumor Necrosis Factor-alpha/*physiology
MH  - Vascular Cell Adhesion Molecule-1/physiology
EDAT- 2007/10/25 09:00
MHDA- 2007/12/21 09:00
CRDT- 2007/10/25 09:00
PHST- 2007/10/25 09:00 [pubmed]
PHST- 2007/12/21 09:00 [medline]
PHST- 2007/10/25 09:00 [entrez]
PST - ppublish
SO  - Rhinology. 2007 Sep;45(3):235-41.