PMID- 17957661
OWN - NLM
STAT- MEDLINE
DCOM- 20080206
LR  - 20071116
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 28
IP  - 22
DP  - 2007 Nov
TI  - Multiple pesticide analysis in wine by MEKC combined with solid-phase 
      microextraction and sample stacking.
PG  - 4072-81
AB  - In this work, a new method for the determination in white wines of 12 pesticides 
      widely used in vine cultivars (namely, carbendazim, pirimicarb, metalaxyl, 
      pyrimethanil, procymidone, nuarimol, azoxystrobin, tebufenozide, fenarimol, 
      benalaxyl, penconazole, and tetradifon) using solid-phase microextraction (SPME) 
      and MEKC with diode-array detection (DAD) was developed. The MEKC buffer 
      consisted of 100 mM sodium tetraborate and 30 mM SDS at pH 8.5 with 6% v/v 
      1-propanol. Reversed-electrode polarity stacking mode (REPSM) was applied as 
      on-line preconcentration strategy. In order to carry out an effective and 
      sensitive determination of these pesticides in wine samples, an off-line SPME 
      procedure was optimized by means of an experimental design. After studying the 
      extraction performance of different SPME coatings, PDMS/divinylbenzene (PDMS/DVB) 
      fibers were found the most appropriate for the extraction of most of these 
      pesticides. Carbendazim and metalaxyl could not be extracted from wine samples. 
      Calibration curves for extracted standards and fortified white wines were studied 
      in order to determine the presence of a matrix effect. The combination of both 
      preconcentration procedures (SPME and REPSM) allowed the determination of ten of 
      these pesticides in white wines at concentrations between 0.054 and 0.113 mg/L. 
      (i.e., levels well below the maximum residue limits (MRLs) allowed for these 
      compounds in wine grapes). Ten homemade wines were they analyzed with the 
      optimized method demonstrating the usefulness of the proposed procedure.
FAU - Ravelo-Perez, Lidia M
AU  - Ravelo-Perez LM
AD  - Department of Analytical Chemistry, Nutrition and Food Science, University of La 
      Laguna, La Laguna, Tenerife, Canary Islands, Spain.
FAU - Hernandez-Borges, Javier
AU  - Hernandez-Borges J
FAU - Borges-Miquel, Teresa M
AU  - Borges-Miquel TM
FAU - Rodriguez-Delgado, Miguel A
AU  - Rodriguez-Delgado MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Pesticide Residues)
RN  - 0 (Pesticides)
SB  - IM
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Food Contamination
MH  - Maximum Allowable Concentration
MH  - Pesticide Residues/analysis
MH  - Pesticides/*analysis/toxicity
MH  - Solid Phase Microextraction
MH  - Vitis/toxicity
MH  - Wine/*analysis/toxicity
EDAT- 2007/10/25 09:00
MHDA- 2008/02/07 09:00
CRDT- 2007/10/25 09:00
PHST- 2007/10/25 09:00 [pubmed]
PHST- 2008/02/07 09:00 [medline]
PHST- 2007/10/25 09:00 [entrez]
AID - 10.1002/elps.200700251 [doi]
PST - ppublish
SO  - Electrophoresis. 2007 Nov;28(22):4072-81. doi: 10.1002/elps.200700251.