PMID- 17957780
OWN - NLM
STAT- MEDLINE
DCOM- 20080919
LR  - 20080616
IS  - 0733-2467 (Print)
IS  - 0733-2467 (Linking)
VI  - 27
IP  - 5
DP  - 2008
TI  - Estrogen-sensitive projections from the medial preoptic area to the dorsal 
      pontine tegmentum, including Barrington's nucleus, in the rat.
PG  - 440-5
AB  - AIM: Urinary incontinence affects a significant number of post-menopausal women. 
      There is conflicting evidence whether voiding symptoms in these women are related 
      to hypoestrogenism or aging itself. This neuroanatomical study was designed to 
      determine whether a specific central nervous system (CNS) pathway that projects 
      to the pontine micturition center (PMC, also known as "Barrington's nucleus") is 
      estrogen sensitive in a rat model. METHODS: A fluorescent retrograde tracer was 
      injected into the dorsal pontine tegmentum of adult female Sprague-Dawley rats to 
      identify neurons in the medial preoptic area (MPA) that project to the PMC. 
      Immunohistochemistry was performed using antibodies directed against estrogen 
      receptor-alpha (ERalpha) and estrogen receptor-beta (ERbeta) to identify 
      estrogen-sensitive neurons. The brain sections were examined using fluorescence 
      microscopy to identify cells that project to the PMC (contain fluorescent tracer) 
      and also express ER (are immunoreactive for ER). RESULTS: There are neurons in 
      the MPA that are double labeled (contain fluorescent tracer and express ERalpha, 
      but not ERbeta), showing that a subset of neurons projecting from the MPA to the 
      PMC is estrogen sensitive. CONCLUSIONS: A subset of estrogen-sensitive neurons 
      projects from the MPA to the PMC in rats, raising the possibility that indirect 
      estrogenic regulation of forebrain neuronal function may modulate the micturition 
      reflex. Future development of drugs that alter the function of this 
      estrogen-sensitive CNS pathway may provide therapeutic strategies to treat 
      post-menopausal incontinence.
FAU - Rickey, Leslie Minor
AU  - Rickey LM
AD  - Division of Urology, University of Maryland Medical Center, Baltimore, Maryland 
      21201, USA. lrickey@smail.umaryland.edu
FAU - Sarkey, Sara
AU  - Sarkey S
FAU - DonCarlos, Lydia L
AU  - DonCarlos LL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Neurourol Urodyn
JT  - Neurourology and urodynamics
JID - 8303326
RN  - 0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Estrogens)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Stilbamidines)
SB  - IM
MH  - Animals
MH  - Data Interpretation, Statistical
MH  - Estrogen Receptor alpha/drug effects/metabolism
MH  - Estrogen Receptor beta/drug effects/metabolism
MH  - Estrogens/*physiology
MH  - Female
MH  - Fluorescent Dyes
MH  - Locus Coeruleus/*physiology
MH  - Microscopy, Fluorescence
MH  - Neural Pathways/*physiology
MH  - Neurons/metabolism
MH  - Ovariectomy
MH  - Pons/*physiology
MH  - Preoptic Area/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stilbamidines
EDAT- 2007/10/25 09:00
MHDA- 2008/09/20 09:00
CRDT- 2007/10/25 09:00
PHST- 2007/10/25 09:00 [pubmed]
PHST- 2008/09/20 09:00 [medline]
PHST- 2007/10/25 09:00 [entrez]
AID - 10.1002/nau.20522 [doi]
PST - ppublish
SO  - Neurourol Urodyn. 2008;27(5):440-5. doi: 10.1002/nau.20522.