PMID- 17958543
OWN - NLM
STAT- MEDLINE
DCOM- 20080107
LR  - 20171116
IS  - 0041-1132 (Print)
IS  - 0041-1132 (Linking)
VI  - 47
IP  - 11
DP  - 2007 Nov
TI  - Rapid and effective CD3 T-cell depletion with a magnetic cell sorting program to 
      produce peripheral blood progenitor cell products for haploidentical 
      transplantation in children and adults.
PG  - 2134-42
AB  - BACKGROUND: Effective T-cell depletion is a prerequisite for haploidentical 
      peripheral blood progenitor cell (PBPC) transplantation. This study was performed 
      to investigate the performance of magnetic cell sorting-based direct large-scale 
      T-cell depletion, which is an attractive alternative to standard PBPC enrichment 
      procedures. STUDY DESIGN AND METHODS: PBPCs were harvested from 11 human 
      leukocyte antigen (HLA)-haploidentical donors. T cells labeled with 
      anti-CD3-coated beads were depleted with a commercially available magnetic 
      separation unit (CliniMACS, Miltenyi Biotec) with either the Depletion 2.1 (D2.1, 
      n=11) or the novel Depletion 3.1 (D3.1, n=12) program. If indicated, additional 
      CD34+ selections were performed (n=6). Eleven patients received T-cell-depleted 
      grafts after reduced-intensity conditioning. RESULTS: The median log T-cell 
      depletion was better with the D2.1 compared to the D3.1 (log 3.6 vs. log 2.3, 
      p<0.05) and was further improved by introducing an immunoglobulin G 
      (IgG)-blocking step (log 4.5 and log 3.4, respectively). The D3.1 was superior to 
      the D2.1 (p<0.05) in median recovery of CD34+ cells (90% vs. 78%) and in median 
      recovery of CD3- cells (87% vs. 76%). The median processing times per 10(10) 
      total cells were 0.90 hours (D2.1) and 0.35 hours (D3.1). The transplanted grafts 
      (directly T-cell-depleted products with or without positively selected CD34+ 
      cells) contained a median of 10.5 x 10(6) per kg CD34+, 0.93x10(5) per kg CD3+, 
      and 11.6x10(6) per kg CD56+. Rapid engraftment was achieved in 10 patients. The 
      incidences of acute graft-versus-host disease were less than 10 percent (Grade 
      I/II) and 0 percent (Grade III/IV). CONCLUSION: The novel D3.1 program with IgG 
      blocking enables highly effective, time-saving large-scale T-cell depletion. 
      Combining direct depletion techniques with standard CD34+ selection enables the 
      composition of grafts optimized to the specific requirements of the patients.
FAU - Dykes, Josefina H
AU  - Dykes JH
AD  - Blood Center, the Department of Pediatric Oncology, Lund University Hospital, 
      Lund, Sweden. josefina.dykes@med.lu.se
FAU - Toporski, Jacek
AU  - Toporski J
FAU - Juliusson, Gunnar
AU  - Juliusson G
FAU - Bekassy, Albert N
AU  - Bekassy AN
FAU - Lenhoff, Stig
AU  - Lenhoff S
FAU - Lindmark, Anders
AU  - Lindmark A
FAU - Scheding, Stefan
AU  - Scheding S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Antigens, CD34)
RN  - 0 (CD3 Complex)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, CD34
MH  - *CD3 Complex
MH  - Child
MH  - Child, Preschool
MH  - Haplotypes
MH  - Hematopoietic Stem Cells
MH  - Histocompatibility Testing
MH  - Humans
MH  - Immunomagnetic Separation
MH  - Immunophenotyping
MH  - Lymphocyte Depletion/*methods
MH  - Middle Aged
MH  - Peripheral Blood Stem Cell Transplantation/*methods
MH  - Transplantation, Homologous
MH  - Treatment Outcome
EDAT- 2007/10/26 09:00
MHDA- 2008/01/08 09:00
CRDT- 2007/10/26 09:00
PHST- 2007/10/26 09:00 [pubmed]
PHST- 2008/01/08 09:00 [medline]
PHST- 2007/10/26 09:00 [entrez]
AID - TRF01438 [pii]
AID - 10.1111/j.1537-2995.2007.01438.x [doi]
PST - ppublish
SO  - Transfusion. 2007 Nov;47(11):2134-42. doi: 10.1111/j.1537-2995.2007.01438.x.