PMID- 17959053
OWN - NLM
STAT- MEDLINE
DCOM- 20090514
LR  - 20131121
IS  - 0253-9624 (Print)
IS  - 0253-9624 (Linking)
VI  - 41
IP  - 4
DP  - 2007 Jul
TI  - [Effects of zearalenone on the proliferation of SK-N-SH human neuroblastoma 
      cells].
PG  - 295-8
AB  - OBJECTIVE: To investigate effects of zearalenone (ZEA) on the proliferation of 
      SK-N-SH human neuroblastoma cells in vitro and its possible mechanism. METHODS: 
      SK-N-SH cells were cultured in estrogen-free improved minimum essential medium 
      and divided into 5 groups based on different treatments: group 1, without 
      treatment; group 2, treated with 17beta-estradiol (E(2)); group 3, treated with 
      ZEA; group 4, treated with both E(2) and ICI 182780; group 5, treated with both 
      ZEA and ICI 182780. Absorbance value (AV) was determined at the time point of 0, 
      24, 48 and 72 hours, and DNA proliferation index (PI) at 72 hours. Flow 
      cytometer, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling 
      (TUNEL) were employed to monitor cell apoptosis. RESULTS: At 24, 48 and 72 hours, 
      the AV of group 3 were 1.39, 1.32, and 1.22 times to those of group 1, 
      respectively. PI in group 3 was 1.43 times of that in group 1 at 72 hours. The 
      results of group 2 were similar to those in group 3. At the same time, the growth 
      of cells was inhibited by ICI 182780 despite the presence of E(2) and ZEA. 
      Apoptosis cells were abundant in group 1 and ICI 182780 groups, but little in 
      E(2) and ZEA groups. CONCLUSION: ZEA might promote the proliferation of SK-N-SH 
      cells to a level similar to that of E(2), which might probably be brought about 
      via estrogen receptor pathways and depressing apoptosis.
FAU - Zheng, Ji-cui
AU  - Zheng JC
AD  - Department of Surgery, Children's Hospital of Fudan University, Shanghai 200032, 
      China.
FAU - Xiao, Xian-min
AU  - Xiao XM
FAU - Zheng, Shan
AU  - Zheng S
FAU - Dong, Kui-ran
AU  - Dong KR
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yu Fang Yi Xue Za Zhi
JT  - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
JID - 7904962
RN  - 0 (Receptors, Estrogen)
RN  - 5W827M159J (Zearalenone)
SB  - IM
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Humans
MH  - Neuroblastoma
MH  - Receptors, Estrogen/antagonists & inhibitors
MH  - Zearalenone/*toxicity
EDAT- 2007/10/26 09:00
MHDA- 2009/05/15 09:00
CRDT- 2007/10/26 09:00
PHST- 2007/10/26 09:00 [pubmed]
PHST- 2009/05/15 09:00 [medline]
PHST- 2007/10/26 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yu Fang Yi Xue Za Zhi. 2007 Jul;41(4):295-8.