PMID- 17959074
OWN - NLM
STAT- MEDLINE
DCOM- 20081023
LR  - 20140226
IS  - 0578-1426 (Print)
IS  - 0578-1426 (Linking)
VI  - 46
IP  - 7
DP  - 2007 Jul
TI  - [Expression of high mobility group box chromosomal protein 1 in peripheral blood 
      of patients with rheumatoid arthritis].
PG  - 547-50
AB  - OBJECTIVE: To investigate the mRNA and protein expression of high mobility group 
      box chromosomal protein 1 (HMGB1) in peripheral blood mononuclear cells (PBMCs) 
      and serum. METHODS: Levels of HMGB1mRNA were detected with reverse transcription 
      polymerase chain reaction (RT-PCR) in PBMC and levels of HMGB1 protein in PBMC 
      and plasm were measured with Western blot in 38 patients with active rheumatoid 
      arthritis (RA), 24 with inactive RA and 20 healthy controls. Ficoll density 
      gradient centrifugation was used to separate PBMCs from peripheral blood. The 
      correlation between the levels of HMGB1 in serum and the index of disease 
      activity in RA was analyzed. RESULTS: There was no statistically significant 
      difference of the mRNA expression of HMGB1 in PBMCs from active RA patients as 
      compared with those from inactive RA group and healthy controls (F = 1.23, P > 
      0.05). The protein expression of HMGB1 was significantly lower in PBMCs from 
      active RA patients (F = 70.91, P < 0.01), while the protein expression of HMGB1 
      was higher in plasma from active RA patients (P < 0.001) as compared with that 
      from inactive RA patients and healthy controls. However, there was no 
      statistically significant difference between inactive RA patients and healthy 
      controls (P > 0.05). The level of HMGB1 protein in plasma of RA patients was 
      correlated with erythrocyte sedimentation rate (ESR) (r(s) = 0.478, P < 0.001) 
      and C- reactive protein (CRP) (r(s) = 0.574, P < 0.05). It was not correlated 
      with age, the number of tender joints and swollen joints, radiographic scores and 
      therapeutic effect. CONCLUSION: The protein expression of HMGB1 was significantly 
      decreased in PBMCs from active RA patients, while it was increased in serum from 
      active RA patients. HMGB1 plays a pivotal role in the pathogenesis of RA and may 
      be a target of therapy as a novel cytokine.
FAU - Zuo, Xiao-xia
AU  - Zuo XX
AD  - Department of Rheumatology and Clinical Immunology, Xiangya Hospital, Central 
      South University, Changsha 410008, China. susanzuo@hotmail.com
FAU - Zhou, Ya-ou
AU  - Zhou YO
FAU - Gong, Yan-hui
AU  - Gong YH
FAU - Wang, Yan-ping
AU  - Wang YP
FAU - Tang, Dao-lin
AU  - Tang DL
FAU - Xiao, Xian-zhong
AU  - Xiao XZ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Nei Ke Za Zhi
JT  - Zhonghua nei ke za zhi
JID - 16210490R
RN  - 0 (HMGB1 Protein)
RN  - 0 (RNA, Messenger)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Adult
MH  - Arthritis, Rheumatoid/*blood/pathology
MH  - Blotting, Western
MH  - C-Reactive Protein/metabolism
MH  - Female
MH  - Gene Expression
MH  - HMGB1 Protein/*blood/genetics/metabolism
MH  - Humans
MH  - Leukocytes, Mononuclear/cytology/metabolism
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2007/10/26 09:00
MHDA- 2008/10/24 09:00
CRDT- 2007/10/26 09:00
PHST- 2007/10/26 09:00 [pubmed]
PHST- 2008/10/24 09:00 [medline]
PHST- 2007/10/26 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Nei Ke Za Zhi. 2007 Jul;46(7):547-50.