PMID- 17959664 OWN - NLM STAT- MEDLINE DCOM- 20080110 LR - 20231105 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 82 IP - 1 DP - 2008 Jan TI - Identification and classification of endogenous retroviruses in cattle. PG - 582-7 AB - The aim of this study was to identify the endogenous retrovirus (ERV) sequences in a bovine genome. We subjected bovine genomic DNA to PCR with degenerate or ovine ERV (OERV) family-specific primers that aimed to amplify the retroviral pro/pol region. Sequence analysis of 113 clones obtained by PCR revealed that 69 were of retroviral origin. On the basis of the OERV classification system, these clones from degenerate PCR could be divided into the beta3, gamma4, and gamma9 families. PCR with OERV family-specific primers revealed an additional ERV that was classified into the bovine endogenous retrovirus (BERV) gamma7 family. In conclusion, here we report the results of a genome scale study of the BERV. Our study shows that the ERV family expansion in cattle may be somewhat limited, while more diverse family members of ERVs have been reported from other artiodactyls, such as pigs and sheep. FAU - Xiao, Rui AU - Xiao R AD - Department of Animal Biotechnology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 143-701, South Korea. chankyu@konkuk.ac.kr. FAU - Park, Kwangha AU - Park K FAU - Lee, Hoontaek AU - Lee H FAU - Kim, Jinhoi AU - Kim J FAU - Park, Chankyu AU - Park C LA - eng SI - GENBANK/DQ889607 SI - GENBANK/DQ889608 SI - GENBANK/DQ889609 SI - GENBANK/DQ889610 SI - GENBANK/DQ889611 SI - GENBANK/DQ889612 SI - GENBANK/DQ889613 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20071024 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (DNA, Viral) RN - 9007-49-2 (DNA) SB - IM MH - Animals MH - Cattle/*virology MH - Cluster Analysis MH - DNA/genetics MH - DNA, Viral/genetics/*isolation & purification MH - Endogenous Retroviruses/*classification/genetics/*isolation & purification MH - Genome/genetics MH - Molecular Sequence Data MH - Phylogeny MH - Polymerase Chain Reaction MH - Sequence Analysis, DNA PMC - PMC2224374 EDAT- 2007/10/26 09:00 MHDA- 2008/01/11 09:00 PMCR- 2008/05/01 CRDT- 2007/10/26 09:00 PHST- 2007/10/26 09:00 [pubmed] PHST- 2008/01/11 09:00 [medline] PHST- 2007/10/26 09:00 [entrez] PHST- 2008/05/01 00:00 [pmc-release] AID - JVI.01451-07 [pii] AID - 1451-07 [pii] AID - 10.1128/JVI.01451-07 [doi] PST - ppublish SO - J Virol. 2008 Jan;82(1):582-7. doi: 10.1128/JVI.01451-07. Epub 2007 Oct 24.