PMID- 17959666
OWN - NLM
STAT- MEDLINE
DCOM- 20080110
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 82
IP  - 1
DP  - 2008 Jan
TI  - Yaba monkey tumor virus encodes a functional inhibitor of interleukin-18.
PG  - 522-8
AB  - Interleukin-18 (IL-18) is a critical proinflammatory cytokine whose extracellular 
      bioactivity is regulated by a cellular IL-18 binding protein (IL-18BP). Many 
      poxviruses have acquired variants of this IL-18BP gene, some of which have been 
      shown to act as viral virulence factors. Yaba monkey tumor virus (YMTV) encodes a 
      related family member, 14L, which is similar to the orthopoxvirus IL-18BPs. YMTV 
      14L was expressed from a baculovirus system and tested for its ability to bind 
      and inhibit IL-18. We found that YMTV 14L bound both human IL-18 (hIL-18) and 
      murine IL-18 with high affinity, at 4.1 nM and 6.5 nM, respectively. YMTV 14L was 
      able to fully sequester hIL-18 but could only partially inhibit the biological 
      activity of hIL-18 as measured by gamma interferon secretion from KG-1 cells. 
      Additionally, 17 hIL-18 point mutants were tested by surface plasmon resonance 
      for their ability to bind to YMTV 14L. Two clusters of hIL-18 surface residues 
      were found to be important for the hIL-18-YMTV 14L interaction, in contrast to 
      results for the Variola virus IL-18BP, which has been shown to primarily interact 
      with a single cluster of three amino acids. The altered binding specificity of 
      YMTV 14L most likely represents an adaptation resulting in increased fitness of 
      the virus and affirms the plasticity of poxviral inhibitor domains that target 
      cytokines like IL-18.
FAU - Nazarian, Steven H
AU  - Nazarian SH
AD  - University of Florida, 1600 SW Archer Road, ARB Room R4-295, P.O. Box 100332, 
      Gainesville, FL 32610, USA.
FAU - Rahman, Masmudur M
AU  - Rahman MM
FAU - Werden, Steven J
AU  - Werden SJ
FAU - Villeneuve, Danielle
AU  - Villeneuve D
FAU - Meng, Xiangzhi
AU  - Meng X
FAU - Brunetti, Craig
AU  - Brunetti C
FAU - Valeriano, Chalice
AU  - Valeriano C
FAU - Wong, Christina
AU  - Wong C
FAU - Singh, Rajkumari
AU  - Singh R
FAU - Barrett, John W
AU  - Barrett JW
FAU - Xiang, Yan
AU  - Xiang Y
FAU - McFadden, Grant
AU  - McFadden G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071024
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (Viral Proteins)
RN  - 0 (interleukin-18 binding protein)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cell Line
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*physiology
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-18/*antagonists & inhibitors
MH  - Mutagenesis, Site-Directed
MH  - Point Mutation
MH  - Protein Binding
MH  - Protein Interaction Mapping
MH  - Surface Plasmon Resonance
MH  - Viral Proteins/*physiology
MH  - Yaba monkey tumor virus/*immunology
PMC - PMC2224402
EDAT- 2007/10/26 09:00
MHDA- 2008/01/11 09:00
PMCR- 2008/05/01
CRDT- 2007/10/26 09:00
PHST- 2007/10/26 09:00 [pubmed]
PHST- 2008/01/11 09:00 [medline]
PHST- 2007/10/26 09:00 [entrez]
PHST- 2008/05/01 00:00 [pmc-release]
AID - JVI.00688-07 [pii]
AID - 0688-07 [pii]
AID - 10.1128/JVI.00688-07 [doi]
PST - ppublish
SO  - J Virol. 2008 Jan;82(1):522-8. doi: 10.1128/JVI.00688-07. Epub 2007 Oct 24.