PMID- 17960569
OWN - NLM
STAT- MEDLINE
DCOM- 20080219
LR  - 20211020
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 215
IP  - 1
DP  - 2008 Apr
TI  - Systemically transplanted bone marrow stromal cells contributing to bone tissue 
      regeneration.
PG  - 204-9
AB  - Bone marrow stromal cells (BMSCs) are a rich source of osteogenic progenitor 
      cells. A fundamental question is whether systemically transplanted BMSCs 
      participate in bone regeneration. Luciferase and GFP double-labeled BMSCs were 
      transplanted into irradiated mice. Five weeks after transplantation, artificial 
      bone wounds were created in the mandibles and calvaria of the recipients. Animals 
      were sacrificed at weeks 2, 4, and 6 after surgery and the expressions of 
      luciferase and GFP were determined using Xenogen IVIS Imaging System, 
      immunohistochemical staining and RT-PCR. The results demonstrated that 
      transplanted BMSCs can be detected in wound sites as early as 2 weeks and lasted 
      the whole experimental period. Luciferase expression peaked at 2 weeks after 
      surgery and decreased thereafter, exhibiting a similar expression pattern as that 
      of BSP, while GFP expression was relatively stable during the experimental 
      period. In conclusion, BMSCs can migrate to bone wound sites and participate in 
      bone regeneration in orocraniofacial region.
CI  - (c) 2007 Wiley-Liss, Inc.
FAU - Li, S
AU  - Li S
AD  - Division of Oral Biology, Department of General Dentistry, Tufts University 
      School of Dental Medicine, Boston, Massachusetts 02111, USA.
FAU - Tu, Q
AU  - Tu Q
FAU - Zhang, J
AU  - Zhang J
FAU - Stein, G
AU  - Stein G
FAU - Lian, J
AU  - Lian J
FAU - Yang, P S
AU  - Yang PS
FAU - Chen, J
AU  - Chen J
LA  - eng
GR  - DE14537/DE/NIDCR NIH HHS/United States
GR  - DE16710/DE/NIDCR NIH HHS/United States
GR  - R01 DE014537-04/DE/NIDCR NIH HHS/United States
GR  - R01 DE016710-03/DE/NIDCR NIH HHS/United States
GR  - R01 DE014537/DE/NIDCR NIH HHS/United States
GR  - R01 DE016710/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Ibsp protein, mouse)
RN  - 0 (Integrin-Binding Sialoprotein)
RN  - 0 (Sialoglycoproteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - *Bone Regeneration
MH  - Bone and Bones/pathology/*physiology
MH  - Breeding
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression Regulation
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Integrin-Binding Sialoprotein
MH  - Kinetics
MH  - Luciferases/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Sialoglycoproteins/genetics/metabolism
MH  - Skull/pathology
MH  - Stromal Cells/*transplantation
PMC - PMC2828813
MID - NIHMS178782
EDAT- 2007/10/26 09:00
MHDA- 2008/02/20 09:00
PMCR- 2010/02/25
CRDT- 2007/10/26 09:00
PHST- 2007/10/26 09:00 [pubmed]
PHST- 2008/02/20 09:00 [medline]
PHST- 2007/10/26 09:00 [entrez]
PHST- 2010/02/25 00:00 [pmc-release]
AID - 10.1002/jcp.21302 [doi]
PST - ppublish
SO  - J Cell Physiol. 2008 Apr;215(1):204-9. doi: 10.1002/jcp.21302.