PMID- 17961122
OWN - NLM
STAT- MEDLINE
DCOM- 20080410
LR  - 20111117
IS  - 1470-8736 (Electronic)
IS  - 0143-5221 (Linking)
VI  - 114
IP  - 7
DP  - 2008 Apr
TI  - Plasma proteome changes in subjects with Type 2 diabetes mellitus with a low or 
      high early insulin response.
PG  - 499-507
AB  - Circulating proteins contribute to the pathogenesis of T2DM (Type 2 diabetes 
      mellitus) in various ways. The aim of the present study was to investigate 
      variations in plasma protein levels in subjects with T2DM and differences in 
      beta-cell function, characterized by the EIR (early insulin response), and to 
      compare these protein levels with those observed in individuals with NGT (normal 
      glucose tolerance). Ten subjects with NGT+high EIR, ten with T2DM+high EIR, and 
      ten with T2DM+low EIR were selected from the community-based ULSAM (Uppsala 
      Longitudinal Study of Adult Men) cohort. Plasma protein profiling was performed 
      using SELDI-TOF (surface-enhanced laser-desorption ionization-time-of-flight) MS. 
      In total, nine plasma proteins differed between the three study groups (P<0.05, 
      as determined by ANOVA). The levels of two forms of transthyretin, haemoglobin 
      alpha-chain and haemoglobin beta-chain were decreased in plasma from subjects 
      with T2DM compared with subjects with NGT, irrespective of the EIR of the 
      subjects. Apolipoprotein H was decreased in plasma from individuals with 
      T2DM+high EIR compared with subjects with NGT. Four additional unidentified 
      plasma proteins also varied in different ways between the experimental groups. In 
      conclusion, the proteins detected in the present study may be related to the 
      development of beta-cell dysfunction.
FAU - Sundsten, Tea
AU  - Sundsten T
AD  - Department of Medical Cell Biology, Uppsala University, SE-751 23 Uppsala, 
      Sweden. Tea.Sundsten@mcb.uu.se
FAU - Zethelius, Bjorn
AU  - Zethelius B
FAU - Berne, Christian
AU  - Berne C
FAU - Bergsten, Peter
AU  - Bergsten P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Sci (Lond)
JT  - Clinical science (London, England : 1979)
JID - 7905731
RN  - 0 (Blood Glucose)
RN  - 0 (Blood Proteins)
RN  - 0 (Insulin)
RN  - 0 (Proteome)
RN  - 9035-68-1 (Proinsulin)
SB  - IM
MH  - Aged
MH  - Blood Glucose/metabolism
MH  - Blood Proteins/*metabolism
MH  - Cross-Sectional Studies
MH  - Diabetes Mellitus, Type 2/*blood/physiopathology
MH  - Follow-Up Studies
MH  - Glucose Tolerance Test
MH  - Humans
MH  - Insulin/*biosynthesis
MH  - Insulin Resistance
MH  - Insulin-Secreting Cells/physiology
MH  - Male
MH  - Proinsulin/blood
MH  - Proteome
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
EDAT- 2007/10/27 09:00
MHDA- 2008/04/11 09:00
CRDT- 2007/10/27 09:00
PHST- 2007/10/27 09:00 [pubmed]
PHST- 2008/04/11 09:00 [medline]
PHST- 2007/10/27 09:00 [entrez]
AID - CS20070323 [pii]
AID - 10.1042/CS20070323 [doi]
PST - ppublish
SO  - Clin Sci (Lond). 2008 Apr;114(7):499-507. doi: 10.1042/CS20070323.