PMID- 17963241
OWN - NLM
STAT- MEDLINE
DCOM- 20080902
LR  - 20131121
IS  - 1932-5223 (Print)
IS  - 1932-5223 (Linking)
VI  - 307
IP  - 12
DP  - 2007 Dec 1
TI  - Effects of tumor necrosis factor-alpha on cell proliferation, prostaglandins and 
      matrix-metalloproteinases production in rat endometrial stromal cells cultured in 
      vitro.
PG  - 699-707
AB  - Tumor necrosis factor-alpha (TNF-alpha) is known as a pluripotent cell mediator, 
      and it is implicated in the control of uterine cell growth, differentiation and 
      function during estrous cycle and pregnancy. In this study, we investigated the 
      effect of TNF-alpha on endometrial stromal cells derived from rat uterus (rat 
      endometrial stromal cells, RES). RES were isolated from rat endometrium at day 5 
      of pregnancy. Proliferation activities of RES were measured by using 
      bromodeoxyuridine (BrdU) labeling kit, the productions of prostaglandin E2 (PGE2) 
      and prostaglandin F2alpha (PGF2alpha) were measured by enzyme immunoassay kits 
      and the production of matrix metalloproteinases (MMPs) was analyzed by 
      gelatin-zymography. TNF-alpha, as well as epidermal growth factor and fibroblast 
      growth factor-2, significantly increased the proliferation activity of RES 
      (P<0.05). TNF-alpha selectively stimulated the production of PGE2 in RES 
      (P<0.05), but not the production of PGF2alpha. Additionally, TNF-alpha did not 
      stimulate the production of MMPs in RES at the concentration of 5 ng/mL, compared 
      with the control groups (P>0.05). In conclusion, this study demonstrates several 
      regulational functions of TNF-alpha on RES using in vitro culture system. The 
      effects of TNF-alpha on proliferation and MMP production of RES have been shown 
      for the first time. We believe that these results demonstrate part of the 
      functions of TNF-alpha in endometrium and contribute to the better understanding 
      of endometrial functions.
FAU - Gamo, Toru
AU  - Gamo T
AD  - Laboratory of Reproductive Physiology and Biotechnology, Department of Animal and 
      Marine Bioresource Sciences, Graduate School, Kyushu University, Fukuoka, Japan.
FAU - Yamauchi, Nobuhiko
AU  - Yamauchi N
FAU - Nishimura, Kyohei
AU  - Nishimura K
FAU - Watanabe, Ryo
AU  - Watanabe R
FAU - Matsumoto, Kenji
AU  - Matsumoto K
FAU - Oozono, Shinji
AU  - Oozono S
FAU - Kubota, Kaiyu
AU  - Kubota K
FAU - He, Pei-Jian
AU  - He PJ
FAU - Soh, Tomoki
AU  - Soh T
FAU - Hattori, Masa-Aki
AU  - Hattori MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Zool A Ecol Genet Physiol
JT  - Journal of experimental zoology. Part A, Ecological genetics and physiology
JID - 101297745
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - B7IN85G1HY (Dinoprost)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Dinoprost/*metabolism
MH  - Dinoprostone/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Embryo, Mammalian/cytology
MH  - Endometrium/*drug effects/metabolism/pathology
MH  - Epidermal Growth Factor/pharmacology
MH  - Female
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Male
MH  - Matrix Metalloproteinases/*metabolism
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Stromal Cells/drug effects/metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2007/10/30 09:00
MHDA- 2008/09/03 09:00
CRDT- 2007/10/30 09:00
PHST- 2007/10/30 09:00 [pubmed]
PHST- 2008/09/03 09:00 [medline]
PHST- 2007/10/30 09:00 [entrez]
AID - 10.1002/jez.a.423 [doi]
PST - ppublish
SO  - J Exp Zool A Ecol Genet Physiol. 2007 Dec 1;307(12):699-707. doi: 
      10.1002/jez.a.423.