PMID- 17964648
OWN - NLM
STAT- MEDLINE
DCOM- 20080513
LR  - 20080310
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 32
IP  - 5
DP  - 2008 May
TI  - FISH is better than BIOMED-2 PCR to detect IgH/BCL2 translocation in follicular 
      lymphoma at diagnosis using paraffin-embedded tissue sections.
PG  - 737-42
AB  - The most common genetic aberration in follicular lymphoma (FL) is the 
      t(14;18)(q32;q21) translocation that juxtaposes the antiapoptotic BCL2 gene with 
      the promoter of the immunoglobulin heavy chain (IgH) gene. Our aim was to test 
      the usefulness of two different techniques, fluorescence in situ hybridization 
      (FISH) and PCR to detect t(14;18) in FL at diagnosis in paraffin-embedded tissue 
      sections. A total of 51 patients diagnosed of FL were analyzed. FISH was 
      performed with dual color dual fusion commercial probes (VYSIS) and in PCR 
      experiments, the BIOMED-2 primers covering MBR, mcr and 3'MBR regions were 
      applied. FISH showed positivity for the IgH/BCL2 translocation in 96% of patients 
      and PCR in 59% of patients. FISH was able to detect variant translocations 
      involving light chain Ig, or showing variant patterns such as deletions of the 
      IgH portion involved in translocation. In 4% of cases, the IgH/BCL2 translocation 
      was not detected by any of the two techniques tested. Our results show that FISH 
      represents the best technique to detect t(14;18) at diagnosis.
FAU - Espinet, Blanca
AU  - Espinet B
AD  - Servei de Patologia (Laboratori de Citogenetica i Biologia Molecular), Hospital 
      del Mar, IMAS, URNHE, PRBB, Escola de Citologia Hematologica Soledad 
      Woessner-IMAS, Barcelona, Spain. bespinet@imas.imim.es
FAU - Bellosillo, Beatriz
AU  - Bellosillo B
FAU - Melero, Carme
AU  - Melero C
FAU - Vela, Ma Carmen
AU  - Vela MC
FAU - Pedro, Carmen
AU  - Pedro C
FAU - Salido, Marta
AU  - Salido M
FAU - Pijuan, Lara
AU  - Pijuan L
FAU - Florensa, Lourdes
AU  - Florensa L
FAU - Besses, Carles
AU  - Besses C
FAU - Serrano, Sergi
AU  - Serrano S
FAU - Sole, Francesc
AU  - Sole F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071026
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - *Genes, bcl-2
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphoma, Follicular/*genetics
MH  - Male
MH  - Middle Aged
MH  - Paraffin Embedding
MH  - Polymerase Chain Reaction/*methods
MH  - *Translocation, Genetic
EDAT- 2007/10/30 09:00
MHDA- 2008/05/14 09:00
CRDT- 2007/10/30 09:00
PHST- 2007/08/02 00:00 [received]
PHST- 2007/09/17 00:00 [revised]
PHST- 2007/09/19 00:00 [accepted]
PHST- 2007/10/30 09:00 [pubmed]
PHST- 2008/05/14 09:00 [medline]
PHST- 2007/10/30 09:00 [entrez]
AID - S0145-2126(07)00351-7 [pii]
AID - 10.1016/j.leukres.2007.09.010 [doi]
PST - ppublish
SO  - Leuk Res. 2008 May;32(5):737-42. doi: 10.1016/j.leukres.2007.09.010. Epub 2007 
      Oct 26.