PMID- 17964810
OWN - NLM
STAT- MEDLINE
DCOM- 20081105
LR  - 20131121
IS  - 1050-4648 (Print)
IS  - 1050-4648 (Linking)
VI  - 23
IP  - 6
DP  - 2007 Dec
TI  - Gene expression and distribution of antibacterial L-amino acid oxidase in the 
      rockfish Sebastes schlegeli.
PG  - 1178-86
AB  - Antibacterial factors in the epidermal mucus of fish have a potential importance 
      in the first line of the host defense response to bacterial pathogens. We 
      previously isolated a novel antibacterial protein termed SSAP (Sebastes schlegeli 
      antibacterial protein) from the skin mucus of the rockfish S. schlegeli and 
      identified it as a new member of the L-amino acid oxidase (LAO) family. In the 
      present study, the localization of SSAP in S. schlegeli was investigated by 
      reverse transcription (RT)-PCR, quantitative real time RT-PCR, Western blotting 
      and measurements of LAO and antibacterial activities. SSAP mRNA was expressed 
      dominantly in skin and gill and weakly in ovary or kidney as shown by RT-PCR and 
      real time RT-PCR. The quantity of SSAP mRNA in skin varied among the individuals, 
      ranging from 1.1 to 13.9 ng microg(-1) total RNA, although no relationship was 
      found between the size of fish and gene expression. SSAP was exclusively detected 
      in skin and gill by Western blotting using a specific anti-SSAP antiserum. In 
      addition, the extracts of both tissues apparently showed LAO activity and 
      antibacterial activity against Photobacterium damselae subsp. piscicida. This 
      study demonstrates that SSAP is predominantly synthesized in skin and gill and 
      probably functions as an antibacterial LAO in both tissues.
FAU - Kitani, Yoichiro
AU  - Kitani Y
AD  - Department of Food Science and Technology, Tokyo University of Marine Science and 
      Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
FAU - Mori, Tsukasa
AU  - Mori T
FAU - Nagai, Hiroshi
AU  - Nagai H
FAU - Toyooka, Keiko
AU  - Toyooka K
FAU - Ishizaki, Shoichiro
AU  - Ishizaki S
FAU - Shimakura, Kuniyoshi
AU  - Shimakura K
FAU - Shiomi, Kazuo
AU  - Shiomi K
FAU - Nagashima, Yuji
AU  - Nagashima Y
LA  - eng
PT  - Journal Article
DEP - 20070503
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Anti-Bacterial Agents)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Blotting, Western
MH  - Fishes/genetics/*metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*immunology
MH  - Gills/enzymology
MH  - Hydrogen Peroxide/metabolism
MH  - L-Amino Acid Oxidase/genetics/*metabolism/pharmacology
MH  - Photobacterium/drug effects
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Skin/enzymology
EDAT- 2007/10/30 09:00
MHDA- 2008/11/06 09:00
CRDT- 2007/10/30 09:00
PHST- 2007/01/24 00:00 [received]
PHST- 2007/03/27 00:00 [revised]
PHST- 2007/04/13 00:00 [accepted]
PHST- 2007/10/30 09:00 [pubmed]
PHST- 2008/11/06 09:00 [medline]
PHST- 2007/10/30 09:00 [entrez]
AID - S1050-4648(07)00088-5 [pii]
AID - 10.1016/j.fsi.2007.04.005 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2007 Dec;23(6):1178-86. doi: 10.1016/j.fsi.2007.04.005. 
      Epub 2007 May 3.