PMID- 17965270
OWN - NLM
STAT- MEDLINE
DCOM- 20080422
LR  - 20240109
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 19
IP  - 10
DP  - 2007 Oct
TI  - SALT TOLERANCE HOMOLOG2, a B-box protein in Arabidopsis that activates 
      transcription and positively regulates light-mediated development.
PG  - 3242-55
AB  - CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1) and ELONGATED HYPOCOTYL5 (HY5) are two 
      major regulators of light signaling in plants. Here, we identify SALT TOLERANCE 
      HOMOLOG2 (STH2) as a gene that interacts genetically with both of these key 
      regulators. STH2 encodes a B-box-containing protein that interacts physically 
      with HY5 in yeast and in plant cells. Whereas STH2 is uniformly nuclear by 
      itself, it shows a COP1-dependent localization to speckles when coexpressed with 
      COP1. We identified two independent T-DNA insertion lines in STH2. Both alleles 
      are hyposensitive to blue, red, and far-red light. The sth2 mutant, like hy5, 
      shows an enhanced number of lateral roots and accumulates less anthocyanin. 
      Analysis of double mutants between sth2 and hy5 indicates that STH2 has both 
      HY5-dependent and -independent functions. Furthermore, besides partially 
      suppressing the hypocotyl phenotype of dark-grown cop1 alleles, sth2 also 
      suppresses the reduced number of lateral roots and high anthocyanin levels in 
      light-grown cop1 alleles. Interestingly, we found that STH2 can activate 
      transcription. Transient transfection assays in protoplasts using a LUC reporter 
      driven by the chalcone isomerase promoter show that the B-boxes in STH2 and a 
      functional G-box element in the promoter are required for this activity. In 
      conclusion, we have identified STH2, a B-box protein in Arabidopsis thaliana, as 
      a positive regulator of photomorphogenesis and report that the B-box domain plays 
      a direct role in activating transcription in plants.
FAU - Datta, Sourav
AU  - Datta S
AD  - Department of Cell and Molecular Biology, Gothenburg University, 405 30 
      Gothenburg, Sweden.
FAU - Hettiarachchi, Chamari
AU  - Hettiarachchi C
FAU - Johansson, Henrik
AU  - Johansson H
FAU - Holm, Magnus
AU  - Holm M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071026
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Anthocyanins)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (HY5 protein, Arabidopsis)
RN  - 0 (Nuclear Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anthocyanins/metabolism
MH  - Arabidopsis/genetics/*growth & development/radiation effects
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Basic-Leucine Zipper Transcription Factors/genetics/metabolism
MH  - Blotting, Northern
MH  - Cell Nucleus/metabolism
MH  - Gene Expression Regulation, Plant
MH  - Hypocotyl/genetics/growth & development/radiation effects
MH  - *Light
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Nuclear Proteins/genetics/metabolism
MH  - Plant Roots/genetics/growth & development/radiation effects
MH  - Protein Binding
MH  - Seedlings/genetics/growth & development/radiation effects
MH  - Sequence Homology, Amino Acid
MH  - *Transcription, Genetic
MH  - Two-Hybrid System Techniques
PMC - PMC2174709
EDAT- 2007/10/30 09:00
MHDA- 2008/04/23 09:00
PMCR- 2008/10/01
CRDT- 2007/10/30 09:00
PHST- 2007/10/30 09:00 [pubmed]
PHST- 2008/04/23 09:00 [medline]
PHST- 2007/10/30 09:00 [entrez]
PHST- 2008/10/01 00:00 [pmc-release]
AID - tpc.107.054791 [pii]
AID - 054791 [pii]
AID - 10.1105/tpc.107.054791 [doi]
PST - ppublish
SO  - Plant Cell. 2007 Oct;19(10):3242-55. doi: 10.1105/tpc.107.054791. Epub 2007 Oct 
      26.