PMID- 17966695
OWN - NLM
STAT- MEDLINE
DCOM- 20071113
LR  - 20191210
IS  - 1061-4303 (Print)
IS  - 1061-4303 (Linking)
VI  - 79
IP  - 11
DP  - 2007 Oct
TI  - Filamentous scum bacteria in activated sludge plants: detection and 
      identification quality by conventional activated sludge microscopy versus 
      fluorescence in situ hybridization.
PG  - 2274-86
AB  - Detection of filamentous bacteria morphotypes involved in scum formation in 
      activated sludge wastewater treatment plants by conventional sludge microscopy is 
      often doomed to fail because of morphological and taxonomical variations. The aim 
      of this study is to compare detection, identification, and quantification quality 
      of filamentous "scum bacteria" found by conventional activated sludge microscopy 
      and fluorescence in situ hybridization (FISH). In the case of filamentous 
      Microthrix parvicella and Eikelboom morphotypes 1863 and 1851, conventional 
      activated sludge microscopy and FISH results correspond well. In contrast, 
      conventional activated sludge microscopy overlooks nocardioform actinomycete and 
      type 1863 single cells. On the other hand, FISH underestimates filamentous 
      nocardioform actinomycetes and morphotypes 0041/0675 or 0092 because of 
      insufficient cell wall permeability or because of their taxonomic variability, 
      with a resulting inadequacy of previously published probes. Nostocoida limicola 
      morphotype results are still inconclusive because of low bacteria numbers being 
      available in situ and the enormous taxonomic variability within this group.
FAU - Muller, Elisabeth
AU  - Muller E
AD  - Institute of Water Quality Control, Technical University of Munich, Garching, 
      Germany. Lisa.L.Mueller@bv.tum.de
FAU - Schade, Margit
AU  - Schade M
FAU - Lemmer, Hilde
AU  - Lemmer H
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Water Environ Res
JT  - Water environment research : a research publication of the Water Environment 
      Federation
JID - 9886167
RN  - 0 (Sewage)
SB  - IM
MH  - Bacteria/*classification/cytology/isolation & purification
MH  - Classification/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - Microscopy, Phase-Contrast
MH  - Sewage/*microbiology
MH  - Water Purification
EDAT- 2007/10/31 09:00
MHDA- 2007/11/14 09:00
CRDT- 2007/10/31 09:00
PHST- 2007/10/31 09:00 [pubmed]
PHST- 2007/11/14 09:00 [medline]
PHST- 2007/10/31 09:00 [entrez]
AID - 10.2175/106143007x183943 [doi]
PST - ppublish
SO  - Water Environ Res. 2007 Oct;79(11):2274-86. doi: 10.2175/106143007x183943.