PMID- 17967502
OWN - NLM
STAT- MEDLINE
DCOM- 20080320
LR  - 20131121
IS  - 0278-6915 (Print)
IS  - 0278-6915 (Linking)
VI  - 46
IP  - 2
DP  - 2008 Feb
TI  - Anti-clastogenic effect of magnolol on benzo(a)pyrene-induced clastogenicity in 
      mice.
PG  - 694-700
AB  - It was previously reported that magnolol strongly inhibited the mutagenicity 
      induced by the indirect mutagens [benzo(a)pyrene (B(a)P), 
      2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 
      2-aminodipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2), 2-aminoanthracene (2AA), and 
      7,12-dimethylbenz[a]anthracene (DMBA)] in Salmonella typhimurium TA98 and TA100 
      in the Ames test, and that the mechanism of this anti-mutagenic effect may 
      involve the inhibition of the metabolic activation of indirect mutagen enzymes. 
      In this study, the in vivo anti-clastogenic effect of magnolol against 
      clastogenicity induced by B(a)P was evaluated using the micronucleus test in 
      mice. Animals were treated with an oral administration of magnolol (1, 10, and 
      100 mg/kg) at -24, 0, 24, 48, 72, and 96 h before a single intraperitoneal 
      injection of B(a)P. Peripheral blood specimens were prepared 48 h after 
      administration of B(a)P, and analyzed by the acridine orange (AO) technique. The 
      results indicated that magnolol inhibited clastogenicity induced by B(a)P at 
      various administration times. In order to elucidate the mechanism behind this 
      effect, we measured the activity of the detoxifying enzymes 
      [UDP-glucuronosyltransferase (UGT) and glutathione-S-transferase (GST)] and 
      antioxidative enzymes [superoxide dismutase (SOD) and catalase] in the liver when 
      treated with an oral administration of magnolol at various administration times. 
      Its effect on clastogenicity created by exposure to oxidative DNA damage-inducing 
      X-ray irradiation was also evaluated using the micronucleus test in mice. Results 
      showed that magnolol increased the activity of both UGT and SOD enzymes, and also 
      inhibited the clastogenicity induced by X-ray irradiation. Magnolol had an 
      anti-clastogenic effect on B(a)P in the micronucleus test as well as an 
      anti-mutagenic effect on indirect mutagens in the Ames test. The anti-clastogenic 
      effect of magnolol was also suggested by the increases in UGT and SOD enzyme 
      activity, and by the attenuation of oxidative damage induced by X-ray 
      irradiation.
FAU - Saito, Junichiro
AU  - Saito J
AD  - Drug Safety Research Laboratories, Astellas Pharma Inc. 1-8, Azusawa 1-chome, 
      Itabashi-ku, Tokyo 174-8511, Japan. junichiroh.saitoh@jp.astellas.com
FAU - Shibuya, Kiyoshi
AU  - Shibuya K
FAU - Nagase, Hisamitsu
AU  - Nagase H
LA  - eng
PT  - Journal Article
DEP - 20070926
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Lignans)
RN  - 0 (Mutagens)
RN  - 001E35HGVF (magnolol)
RN  - 3417WMA06D (Benzo(a)pyrene)
SB  - IM
MH  - Animals
MH  - Benzo(a)pyrene/*antagonists & inhibitors/toxicity
MH  - Biphenyl Compounds/*pharmacology
MH  - Enzyme Activation/*drug effects
MH  - Lignans/*pharmacology
MH  - Liver/*drug effects/enzymology
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Micronucleus Tests
MH  - Mutagens/*toxicity
EDAT- 2007/10/31 09:00
MHDA- 2008/03/21 09:00
CRDT- 2007/10/31 09:00
PHST- 2006/08/23 00:00 [received]
PHST- 2007/08/27 00:00 [revised]
PHST- 2007/09/17 00:00 [accepted]
PHST- 2007/10/31 09:00 [pubmed]
PHST- 2008/03/21 09:00 [medline]
PHST- 2007/10/31 09:00 [entrez]
AID - S0278-6915(07)00446-2 [pii]
AID - 10.1016/j.fct.2007.09.097 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2008 Feb;46(2):694-700. doi: 10.1016/j.fct.2007.09.097. Epub 
      2007 Sep 26.