PMID- 17969174
OWN - NLM
STAT- MEDLINE
DCOM- 20080326
LR  - 20211020
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 99
IP  - 6
DP  - 2008 Apr 15
TI  - A perfusable 3D cell-matrix tissue culture chamber for in situ evaluation of 
      nanoparticle vehicle penetration and transport.
PG  - 1490-501
AB  - A key factor in gene or drug therapy is the development of carriers that can 
      efficiently reach targeted cells from a distal administration. In many gene/drug 
      delivery studies, results obtained in 2D cultures fail to translate to similar 
      results in vivo. In this work, we developed a perfusable 3D chamber for studying 
      nanoparticle penetration and transport in cell-gel soft tissue cultures. The 
      compartmented chamber is made of a polydimethylsiloxane (PDMS) top layer with the 
      chamber features, created using micromachined lithography, bonded to a bottom 
      glass coverslip. A solution of cells embedded in a hydrogel is loaded in the 
      chamber between PDMS posts that serve as anchors to the cell-matrix at the 
      gel-media interface. The chamber offers the following unique features: (i) rapid 
      fabrication and simplicity in assembly, (ii) direct in situ cell imaging in a 
      plane normal to the direction of flow or action, (iii) an easily configurable and 
      controllable environment conducive cell culture under static or interstitial flow 
      conditions, and (iv) facile recovery of live cells from chambers for 
      post-experimental analysis. To assess the chamber, we delivered fluorescently 
      labeled nanoparticles of three distinct sizes to cells-embedded Matrigels in the 
      3D chamber under flow and static conditions. Penetration of nanoparticles were 
      enhanced under interstitial flow while live cell imaging and flow cytometry of 
      recovered cells revealed particle size restrictions to efficient delivery. 
      Although designed for delivery studies, the chamber is versatile and can be 
      easily modified. Thus it may have broad applications for biological, tissue 
      engineering, and therapeutic studies.
CI  - Copyright 2007 Wiley Periodicals, Inc.
FAU - Ng, Chee Ping
AU  - Ng CP
AD  - Department of Bioengineering, University of Washington, Seattle, Washington, USA.
FAU - Pun, Suzie Hwang
AU  - Pun SH
LA  - eng
GR  - R21 CA114143/CA/NCI NIH HHS/United States
GR  - R21 CA114143-01/CA/NCI NIH HHS/United States
GR  - R21 CA114143-02/CA/NCI NIH HHS/United States
GR  - 1R21CA114141/CA/NCI NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
SB  - IM
MH  - Cell Culture Techniques/*instrumentation/methods
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Flow Cytometry/*instrumentation/methods
MH  - Flow Injection Analysis/*instrumentation/methods
MH  - Microfluidics/*instrumentation/methods
MH  - *Nanoparticles
MH  - Nanotechnology/*instrumentation/methods
MH  - Tissue Culture Techniques/*instrumentation/methods
PMC - PMC2796126
MID - NIHMS122494
EDAT- 2007/10/31 09:00
MHDA- 2008/03/28 09:00
PMCR- 2009/12/18
CRDT- 2007/10/31 09:00
PHST- 2007/10/31 09:00 [pubmed]
PHST- 2008/03/28 09:00 [medline]
PHST- 2007/10/31 09:00 [entrez]
PHST- 2009/12/18 00:00 [pmc-release]
AID - 10.1002/bit.21698 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2008 Apr 15;99(6):1490-501. doi: 10.1002/bit.21698.