PMID- 17970113
OWN - NLM
STAT- MEDLINE
DCOM- 20100610
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 25
IP  - 4
DP  - 2007 Jul
TI  - [Determination of isomerized alpha-acids in beer using solid phase 
      extraction-high performance liquid chromatography].
PG  - 532-5
AB  - An analytical method for four isomerized a-acids in beer was developed using 
      solid phase extraction (SPE) coupled with high performance liquid chromatography 
      (HPLC). The method for solid phase extraction was systematically optimized. The 
      optimal conditions were determined as follows: 2 mL of acidic methanol as eluting 
      solvent through a Sep-Pak C18 cartridge and the pH value of beer was adjusted to 
      2.5. Under these conditions, the recoveries of four isomerized a-acids for spiked 
      standards (n=10) were 90.6%-96.4% and the relative standard deviations (RSDs) 
      were generally less than 4%. The detection limits for iso-alpha-acid, 
      rho-iso-alpha-acid, tetrahydro-iso-alpha-acid and hexahydro-iso-alpha-acid were 
      0.14 mg/L, 0.36 mg/L, 0.33 mg/L and 0.53 mg/L, respectively. The method is 
      sensitive, accurate and suitable for the analysis of isomerized alpha-acids in 
      beer.
FAU - Li, Qi
AU  - Li Q
AD  - Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern 
      Yangtze University, Wuxi 214036, China. liqi@sytu.edu.cn
FAU - Zhou, Tian
AU  - Zhou T
FAU - Gu, Guoxian
AU  - Gu G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Acids)
SB  - IM
MH  - Acids/*analysis/chemistry
MH  - Beer/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Hydrogen-Ion Concentration
MH  - Isomerism
MH  - Limit of Detection
MH  - Solid Phase Extraction/*methods
EDAT- 2007/11/01 09:00
MHDA- 2010/06/11 06:00
CRDT- 2007/11/01 09:00
PHST- 2007/11/01 09:00 [pubmed]
PHST- 2010/06/11 06:00 [medline]
PHST- 2007/11/01 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2007 Jul;25(4):532-5.