PMID- 17970117
OWN - NLM
STAT- MEDLINE
DCOM- 20100610
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 25
IP  - 4
DP  - 2007 Jul
TI  - [Simultaneous determination of vindoline, catharanthine and anhydrovinblastine in 
      Catharanthus roseus by high performance liquid chromatography].
PG  - 550-2
AB  - A high performance liquid chromatographic (HPLC) method with gradient elution was 
      developed for the simultaneous determination of vindoline, catharanthine and 
      anhydrovinblastine in Catharanthus roseus. The analytes were separated on a 
      Waters (5)C18-MS-II column (4.6 mm x 250 mm) and detected at 220 nm. The mobile 
      phases were methanol-1% (v/v) diethylamine solution (adjusted to pH 7.3 with 
      phosphate) for gradient elution at temperature of 25 degrees C. The calibration 
      curves for vindoline, catharanthine and anhydrovinblastine showed good linearity 
      in the ranges of 0.03-1 mg/mL (r = 0.9997), 0.03-1 mg/mL (r = 0.9999) and 
      0.01-0.5 mg/mL (r = 0.9986), respectively. The recoveries were 96.8%, 97.0%, and 
      96.4% and the relative standard deviations (RSDs) were 1.53%, 1.37%, and 1.96%. 
      The method is accurate, rapid and simple for the determination of vindoline, 
      catharanthine and anhydrovinblastine in Catharanthus roseus.
FAU - Yang, Lei
AU  - Yang L
AD  - Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast 
      Forestry University, Harbin 150040, China. alkaloid2004@126.com
FAU - Tang, Zhonghua
AU  - Tang Z
FAU - Zu, Yuangang
AU  - Zu Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Vinca Alkaloids)
RN  - 571PJ1LW03 (vindoline)
RN  - 5V9KLZ54CY (Vinblastine)
RN  - WT0YJV846J (catharanthine)
SB  - IM
MH  - Catharanthus/*chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Tandem Mass Spectrometry
MH  - Vinblastine/*analogs & derivatives/analysis
MH  - Vinca Alkaloids/*analysis
EDAT- 2007/11/01 09:00
MHDA- 2010/06/11 06:00
CRDT- 2007/11/01 09:00
PHST- 2007/11/01 09:00 [pubmed]
PHST- 2010/06/11 06:00 [medline]
PHST- 2007/11/01 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2007 Jul;25(4):550-2.