PMID- 17970118
OWN - NLM
STAT- MEDLINE
DCOM- 20100610
LR  - 20181201
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 25
IP  - 4
DP  - 2007 Jul
TI  - [Simultaneous determination of residues of three fluoroquinolones in chicken 
      using high performance liquid chromatography with post-column derivatization and 
      terbium sensitized fluorescence].
PG  - 553-6
AB  - A method of high performance liquid chromatography with post-column 
      derivatization and terbium sensitized fluorescence for simultaneous determination 
      of residues of ciprofloxacin, norfloxacin and enrofloxacin in chicken was 
      established, based on the effect that Tb3+ can sensibilize fluorescence of 
      fluoroquinolones. Under the optimal chromatographic condition, three 
      fluoroquinolones were separated on a C18 column with 0.05 mol/L acetic 
      acid/acetate buffer-acetonitrile (89:11, v/v) as the mobile phase, and at a flow 
      rate of 0.8 mL/min; then were derivatized with 8 x 10(-5) mol/L Tb3+ at 40 
      degrees C, and at a flow rate of 0.5 mL/min; finally were detected using a 
      fluorescence detector (lamda(ex) = 271 nm, lamda(em) = 545 nm). The recoveries of 
      three fluoroquinolones ranged from 66.3%-88.0% at the added levels of 1.0, 10.0, 
      50.0, and 100.0 ng/g, and the relative standard deviations (RSDs) were less than 
      15.0%. The linear range for quantitative analysis was between 0.1 and 500 ng/mL. 
      All the RSDs of the inter-day and intra-day analyses were less than 13.0%. The 
      detection limits were 0.05 ng/g for ciprofloxacin and norfloxacin and 0.08 ng/g 
      for enrofloxacin. The method is sensitive for the determination of multi-residues 
      of fluoroquinolones in chicken.
FAU - Qi, Kezong
AU  - Qi K
AD  - College of Animal Science and Technology, Anhui Agricultural University, Hefei 
      230036, China. jwccqkz@ahau.edu.cn
FAU - Zhu, Liangqiang
AU  - Zhu L
FAU - Sun, Guoren
AU  - Sun G
FAU - Shi, Zuhao
AU  - Shi Z
FAU - Peng, Kaisong
AU  - Peng K
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Fluoroquinolones)
RN  - 06SSF7P179 (Terbium)
RN  - 3DX3XEK1BN (Enrofloxacin)
RN  - 5E8K9I0O4U (Ciprofloxacin)
RN  - N0F8P22L1P (Norfloxacin)
SB  - IM
MH  - Animals
MH  - Chickens/*metabolism
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Ciprofloxacin/analysis
MH  - Enrofloxacin
MH  - Fluoroquinolones/*analysis
MH  - Norfloxacin/analysis
MH  - Reproducibility of Results
MH  - Terbium/*chemistry
EDAT- 2007/11/01 09:00
MHDA- 2010/06/11 06:00
CRDT- 2007/11/01 09:00
PHST- 2007/11/01 09:00 [pubmed]
PHST- 2010/06/11 06:00 [medline]
PHST- 2007/11/01 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2007 Jul;25(4):553-6.