PMID- 17971303
OWN - NLM
STAT- MEDLINE
DCOM- 20071218
LR  - 20091119
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 364
IP  - 4
DP  - 2007 Dec 28
TI  - Important role of apoptosis signal-regulating kinase 1 in ischemic acute kidney 
      injury.
PG  - 1043-9
AB  - We investigated the role of apoptosis signal-regulating kinase 1 (ASK1) in 
      ischemia/reperfusion (I/R)-induced acute kidney injury (AKI). Blood urea nitrogen 
      (BUN) and serum creatinine were significantly higher in ASK1+/+ mice than in 
      ASK1-/- mice after I/R injury. Renal histology of ASK1+/+ mice showed 
      significantly greater tubular necrosis and degradation. In ASK1-/- mice, 
      phosphorylation of ASK1, JNK, and p38K, and the number of TUNEL-positive cells 
      and infiltrated leukocytes decreased after I/R injury. Apoptotic changes were 
      significantly decreased in cultured renal tubular epithelial cells (TECs) from 
      ASK1-/- mice under hypoxic condition. Transfection with dominant-active ASK1 
      induced apoptosis in TECs. Protein expression of monocyte chemoattractant 
      protein-1 (MCP-1) was significantly weaker in ASK1-/- mice after I/R injury. 
      Transfection with dominant negative-ASK1 significantly decreased MCP-1 production 
      in TECs. These results demonstrated that ASK1 is activated in I/R-induced AKI, 
      and blockage of ASK1 attenuates renal tubular apoptosis, MCP-1 expression, and 
      renal function.
FAU - Terada, Yoshio
AU  - Terada Y
AD  - Department of Nephrology and Blood Purification, Tokyo Medical and Dental 
      University, 5-45, Yushima 1-chome, Bunkyo-ku, Tokyo 113-8519, Japan. 
      yterada.kid@tmd.ac.jp
FAU - Inoshita, Seiji
AU  - Inoshita S
FAU - Kuwana, Hitoshi
AU  - Kuwana H
FAU - Kobayashi, Takahiko
AU  - Kobayashi T
FAU - Okado, Tomokazu
AU  - Okado T
FAU - Ichijo, Hidenori
AU  - Ichijo H
FAU - Sasaki, Sei
AU  - Sasaki S
LA  - eng
PT  - Journal Article
DEP - 20071029
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinase 5)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - Kidney/*blood supply/*metabolism/pathology
MH  - Kidney Tubules/*metabolism/pathology
MH  - MAP Kinase Kinase Kinase 5/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Reperfusion Injury/metabolism/*pathology
EDAT- 2007/11/01 09:00
MHDA- 2007/12/19 09:00
CRDT- 2007/11/01 09:00
PHST- 2007/10/21 00:00 [received]
PHST- 2007/10/22 00:00 [accepted]
PHST- 2007/11/01 09:00 [pubmed]
PHST- 2007/12/19 09:00 [medline]
PHST- 2007/11/01 09:00 [entrez]
AID - S0006-291X(07)02305-4 [pii]
AID - 10.1016/j.bbrc.2007.10.122 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 Dec 28;364(4):1043-9. doi: 
      10.1016/j.bbrc.2007.10.122. Epub 2007 Oct 29.