PMID- 17971925
OWN - NLM
STAT- MEDLINE
DCOM- 20090105
LR  - 20071031
IS  - 1003-9279 (Print)
IS  - 1003-9279 (Linking)
VI  - 21
IP  - 3
DP  - 2007 Sep
TI  - [Immunodominance in CD8+ T cell responses to HIV-1 synthesized epitopes].
PG  - 217-9
AB  - OBJECTIVE: To investigate immunodominance in CD8+ T cell responses to human 
      immunodeficiency virus type 1 (HIV-1) epitopes. METHODS: Frequency of 
      Interferon-gamma (IFN-gamma) secreting cells and the proliferation percentage of 
      CD8+ T cells in PBMC from an HIV-1-infected long term nonprogressor (LTNP) were 
      assessed after stimulation with either the 34 pools of 701 overlapping peptides 
      covering the regions of HIV-1 Env, Pol, Gag, Vif, Nef, Tat or some single 
      peptides, by using various assays including enzyme-linked immunospot (ELISPOT) 
      and CFSE Carboxy-fluorescein diacetate, succinimidyl ester (CFSE) labeling and 
      flow cytometry. RESULTS: HIV-1 Gag peptides induced the highest frequency of 
      IFN-gamma secreting cells, followed by Nef, Tat, and Vif. Meanwhile, Env and Pol 
      failed to induce significant responses. In the IFN-gamma ELISPOT assay, 
      stimulation with single peptide and the corresponsive peptide pool generated 
      analogous results. In addition, the frequencies of IFN-gamma secreting cells and 
      the proliferation percentage of CD8+ T cells detected-ELISPOT and CFSE labeling 
      and flow cytometry were proportional, when single peptides were used for 
      stimulation. CONCLUSION: CD8+ T cells can respond to some specific HIV-1 epitopes 
      and induce immunodominant responses. As a complimentary approach to the standard 
      of ELISPOT assay, We recommend a novel CFSE labeling and flow cytometry assay for 
      the examination of immunodominance in studies of HIV-1 specific proliferation 
      percentage of CD8+ T cell responses.
FAU - Tang, Yang-Bo
AU  - Tang YB
AD  - Institute of infectious diseases, Guangzhou Eighth People's Hospital, Guangzhou, 
      China. yangbotang@tom.com
FAU - Tang, Xiao-Ping
AU  - Tang XP
FAU - Jin, Xia
AU  - Jin X
LA  - chi
PT  - Case Reports
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
JT  - Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang 
      bingduxue zazhi = Chinese journal of experimental and clinical virology
JID - 9602873
RN  - 0 (Immunodominant Epitopes)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - CD8-Positive T-Lymphocytes/drug effects/*immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - HIV Infections/*immunology/virology
MH  - HIV-1/*immunology
MH  - Humans
MH  - Immunodominant Epitopes/*immunology/pharmacology
MH  - Interferon-gamma/metabolism
EDAT- 2007/11/01 09:00
MHDA- 2009/01/06 09:00
CRDT- 2007/11/01 09:00
PHST- 2007/11/01 09:00 [pubmed]
PHST- 2009/01/06 09:00 [medline]
PHST- 2007/11/01 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2007 Sep;21(3):217-9.