PMID- 17973086
OWN - NLM
STAT- MEDLINE
DCOM- 20080501
LR  - 20080124
IS  - 0141-5492 (Print)
IS  - 0141-5492 (Linking)
VI  - 30
IP  - 3
DP  - 2008 Mar
TI  - Label-free homogeneous detection of immunoglobulin E by an aptameric enzyme 
      subunit.
PG  - 421-5
AB  - We have developed an aptameric enzyme subunit (AES) for immunoglobulin E (IgE) 
      sensing. AES is an artificial enzyme subunit constructed from two different 
      aptamers and does not require any modification. Using the AES, the target 
      molecule can be detected by measuring enzymatic activity in homogeneous solution. 
      We connected IgE-binding aptamer and its complementary strand to split 
      thrombin-inhibiting aptamer. The hybrid of these two oligonucleotides inhibited 
      thrombin activity and it decreased in the presence of IgE. We were able to detect 
      IgE by using this AES in homogeneous solution with a detection limit of 50 pmol.
FAU - Yoshida, Wataru
AU  - Yoshida W
AD  - Department of Biotechnology and Life Science, Tokyo University of Agriculture and 
      Technology, Koganei, Tokyo, Japan.
FAU - Sode, Koji
AU  - Sode K
FAU - Ikebukuro, Kazunori
AU  - Ikebukuro K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071031
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
RN  - 0 (Aptamers, Nucleotide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry/metabolism
MH  - Base Sequence
MH  - Biosensing Techniques/*methods
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Thrombin/*antagonists & inhibitors
EDAT- 2007/11/02 09:00
MHDA- 2008/05/02 09:00
CRDT- 2007/11/02 09:00
PHST- 2007/09/03 00:00 [received]
PHST- 2007/10/09 00:00 [accepted]
PHST- 2007/11/02 09:00 [pubmed]
PHST- 2008/05/02 09:00 [medline]
PHST- 2007/11/02 09:00 [entrez]
AID - 10.1007/s10529-007-9575-3 [doi]
PST - ppublish
SO  - Biotechnol Lett. 2008 Mar;30(3):421-5. doi: 10.1007/s10529-007-9575-3. Epub 2007 
      Oct 31.