PMID- 17973830
OWN - NLM
STAT- MEDLINE
DCOM- 20080111
LR  - 20091119
IS  - 1463-5216 (Print)
IS  - 1463-5216 (Linking)
VI  - 10 Suppl 1
DP  - 2007 Nov-Dec
TI  - Retrograde axonal transport obstruction of brain-derived neurotrophic factor 
      (BDNF) and its TrkB receptor in the retina and optic nerve of American Cocker 
      Spaniel dogs with spontaneous glaucoma.
PG  - 12-9
AB  - OBJECTIVE: To determine the degree of retrograde optic nerve axonal transport 
      obstruction at the scleral lamina cribosa level by examining levels of 
      brain-derived neurotrophic factor (BDNF) and its tyrosine kinase receptor type B 
      (TrkB) in dogs presenting with high intraocular pressure. ANIMALS STUDIED: A 
      total of 10 eyes, four normal and six glaucomatous eyes, from normal and affected 
      American Cocker Spaniels with primary glaucoma were studied. All eyes were 
      assessed by neuro-ophthalmic examination, tonometry, gonioscopy, slit-lamp 
      biomicroscopy, and indirect ophthalmoscopy prior to enucleation. METHODS: 
      Immunocytochemistry analysis was performed to evaluate BDNF and TrkB receptor 
      expression in retina and optic nerve in normal and glaucomatous dogs. RESULTS: In 
      all normal eyes BDNF immunostaining was detected in the cytoplasm of retinal 
      ganglion cells (RGC), inner plexiform layer (IPL), inner nuclear layer (INL), 
      nerve fiber layer (NFL), optic nerve head cells, and lamina cribosa cells. In all 
      glaucomatous eyes BDNF was more evident in RGC, NFL and lamina cribosa cells. 
      TrkB receptor was detected in the cytoplasm of RGC, NFL and ONH bundles in all 
      normal eyes, and in a more intense pattern in all glaucomatous eyes. CONCLUSIONS: 
      BDNF retrograde axonal transport is substantially inhibited by intraocular 
      pressure elevation. TrkB accumulation at the ONH in glaucoma suggests a role for 
      neurotrophin deprivation in the pathogenesis of RGC death in canine glaucoma, as 
      well as a possible paracrine and/or autocrine signaling within the lamina 
      cribosa. Neurotrophin signaling may regulate more than neuronal development, 
      survival and differentiation. BDNF neurotrophin and its TrkB receptor expression 
      by lamina cribosa cells and ONH astrocytes in glaucomatous eyes may help to 
      determine the role of these cells as a paracrine source in terms of retinal 
      ganglion cell survival, during episodes of elevated intraocular pressure.
FAU - Iwabe, Simone
AU  - Iwabe S
AD  - Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de 
      Mexico, DF, Mexico.
FAU - Moreno-Mendoza, Norma A
AU  - Moreno-Mendoza NA
FAU - Trigo-Tavera, Francisco
AU  - Trigo-Tavera F
FAU - Crowder, Cheryl
AU  - Crowder C
FAU - Garcia-Sanchez, Gustavo A
AU  - Garcia-Sanchez GA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Ophthalmol
JT  - Veterinary ophthalmology
JID - 100887377
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - *Axonal Transport
MH  - Axons/metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Case-Control Studies
MH  - Dog Diseases/*metabolism
MH  - Dogs
MH  - Female
MH  - Glaucoma/metabolism/*veterinary
MH  - Immunohistochemistry/veterinary
MH  - Male
MH  - Optic Nerve/metabolism
MH  - Pedigree
MH  - Receptor, trkB/*metabolism
MH  - Retinal Ganglion Cells/metabolism
EDAT- 2007/11/29 09:00
MHDA- 2008/01/12 09:00
CRDT- 2007/11/29 09:00
PHST- 2007/11/29 09:00 [pubmed]
PHST- 2008/01/12 09:00 [medline]
PHST- 2007/11/29 09:00 [entrez]
AID - VOP504 [pii]
AID - 10.1111/j.1463-5224.2007.00504.x [doi]
PST - ppublish
SO  - Vet Ophthalmol. 2007 Nov-Dec;10 Suppl 1:12-9. doi: 
      10.1111/j.1463-5224.2007.00504.x.