PMID- 17973924
OWN - NLM
STAT- MEDLINE
DCOM- 20080225
LR  - 20071116
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 26
IP  - 10
DP  - 2007 Nov
TI  - Temporal profile of circadian clock gene expression in a transplanted 
      suprachiasmatic nucleus and peripheral tissues.
PG  - 2731-8
AB  - The mammalian hypothalamic suprachiasmatic nucleus (SCN) is the master oscillator 
      that regulates the circadian rhythms of the peripheral oscillators. Previous 
      studies have demonstrated that the transplantation of embryonic SCN tissues into 
      SCN-lesioned arrhythmic mice restores the behavioral circadian rhythms of these 
      animals. In our present study, we examined the clock gene expression profiles in 
      a transplanted SCN and peripheral tissues, and also analysed the circadian rhythm 
      of the locomotor activity in SCN-grafted mice. These experiments were undertaken 
      to elucidate whether the transplanted SCN generates a dynamic circadian 
      oscillation and maintains the phase relationships that can be detected in intact 
      mice. The grafted SCN indeed showed dynamic circadian expression rhythms of clock 
      genes such as mPeriod1 (mPer1) and mPeriod2 (mPer2). Furthermore, the phase 
      differences between the expression rhythms of these genes in the grafted SCN and 
      the locomotor activity rhythms of the transplanted animals were found to be very 
      similar to those in intact animals. Moreover, in the liver, kidney and skeletal 
      muscles of the transplanted animals, the phase angles between the circadian 
      rhythm of the grafted SCN and that of the peripheral tissues were maintained as 
      in intact animals. However, in the SCN-grafted animals, the amplitudes of the 
      mPer1 and mPer2 rhythms were attenuated in the peripheral tissues. Our current 
      findings therefore indicate that a transplanted SCN has the capacity to generate 
      a dynamic intrinsic circadian oscillation, and can also lock the normal phase 
      angles among the SCN, locomotor activity and peripheral oscillators in a similar 
      manner as in intact control animals.
FAU - Sujino, Mitsugu
AU  - Sujino M
AD  - Department of Anatomy and Neurobiology, Kinki University School of Medicine, 
      377-2 Ohno-Higashi, Osakasayama City, Osaka 589-8511, Japan. 
      sujino@med.kindai.ac.jp
FAU - Nagano, Mamoru
AU  - Nagano M
FAU - Fujioka, Atsuko
AU  - Fujioka A
FAU - Shigeyoshi, Yasufumi
AU  - Shigeyoshi Y
FAU - Inouye, Shin-Ichi T
AU  - Inouye ST
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071031
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Nuclear Proteins)
SB  - IM
MH  - Animals
MH  - Behavior, Animal
MH  - *Brain Tissue Transplantation
MH  - Circadian Rhythm/*physiology
MH  - Embryo, Mammalian
MH  - Gene Expression/*physiology
MH  - Gene Expression Regulation
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Motor Activity/physiology
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Suprachiasmatic Nucleus/injuries/*metabolism/surgery/*transplantation
MH  - Time Factors
EDAT- 2007/11/02 09:00
MHDA- 2008/02/26 09:00
CRDT- 2007/11/02 09:00
PHST- 2007/11/02 09:00 [pubmed]
PHST- 2008/02/26 09:00 [medline]
PHST- 2007/11/02 09:00 [entrez]
AID - EJN5926 [pii]
AID - 10.1111/j.1460-9568.2007.05926.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2007 Nov;26(10):2731-8. doi: 10.1111/j.1460-9568.2007.05926.x. 
      Epub 2007 Oct 31.