PMID- 1797461
OWN - NLM
STAT- MEDLINE
DCOM- 19920416
LR  - 20190903
IS  - 0732-8893 (Print)
IS  - 0732-8893 (Linking)
VI  - 14
IP  - 5
DP  - 1991 Sep-Oct
TI  - Correlative interpretation of staphylococcal resistance to 
      penicillinase-resistant penicillins by ceftizoxime disk susceptibility test using 
      Showa disks.
PG  - 451-5
AB  - The Showa disk susceptibility test (Showa Yakuhin Kako Company, Japan) was 
      evaluated to discriminate between the strains of Staphylococcus aureus resistant 
      to penicillinase-resistant penicillins (PRPs) and those susceptible. When we 
      tested 129 PRP-resistant and 112 susceptible strains, many PRP-resistant strains 
      were interpreted to be false susceptible to methicillin and oxacillin, especially 
      when incubated at 37 degrees C. Growth at 30 degrees C and when NaCl was added to 
      the medium improved the test reliability for detection of PRP resistance. 
      Ceftizoxime disk (30 micrograms) susceptibility test results highly correlated 
      with reference PRP resistance when incubated at 35 degrees C. All the 
      PRP-resistant strains produced no zone of inhibition, whereas all of the 
      PRP-susceptible strains were categorized as susceptible to ceftizoxime (greater 
      than or equal to 22 mm, less than or equal to 3.13 micrograms/ml), for example, 
      100% correlation. It was concluded that the Showa ceftizoxime disk, in 
      replacement of PRPs, will provide a reliable, alternative method to predict PRP 
      resistance in S. aureus pending reevaluation of the Show PRP disk tests.
FAU - Yamane, N
AU  - Yamane N
AD  - Department of Laboratory Medicine, Kumamoto University Medical School, Japan.
FAU - Tosaka, M
AU  - Tosaka M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Diagn Microbiol Infect Dis
JT  - Diagnostic microbiology and infectious disease
JID - 8305899
RN  - 0 (Cephalosporins)
RN  - 0 (Culture Media)
RN  - C43C467DPE (Ceftizoxime)
RN  - EC 3.5.2.- (Penicillinase)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
MH  - Ceftizoxime/*pharmacology
MH  - Cephalosporins/pharmacology
MH  - Culture Media
MH  - Methicillin Resistance
MH  - *Microbial Sensitivity Tests
MH  - Oxacillin/pharmacology
MH  - *Penicillin Resistance
MH  - Penicillinase/*metabolism
MH  - Staphylococcus aureus/*drug effects
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
AID - 0732-8893(91)90073-O [pii]
AID - 10.1016/0732-8893(91)90073-o [doi]
PST - ppublish
SO  - Diagn Microbiol Infect Dis. 1991 Sep-Oct;14(5):451-5. doi: 
      10.1016/0732-8893(91)90073-o.