PMID- 17975261
OWN - NLM
STAT- MEDLINE
DCOM- 20080324
LR  - 20151119
IS  - 1479-6813 (Electronic)
IS  - 0952-5041 (Linking)
VI  - 39
IP  - 5
DP  - 2007 Nov
TI  - Steroidogenic factor 1/adrenal 4 binding protein transforms human bone marrow 
      mesenchymal cells into steroidogenic cells.
PG  - 343-50
AB  - Steroidogenic factor 1/adrenal 4 binding protein (SF-1/Ad4BP) is an essential 
      nuclear receptor for steroidogenesis as well as for adrenal and gonadal gland 
      development. Mesenchymal bone marrow cells (BMCs) contain pluripotent progenitor 
      cells, which differentiate into multiple lineages. In a previous study, we 
      reported that adenovirus-mediated forced expression of SF-1 could transform mouse 
      primary long-term cultured BMCs into steroidogenic cells. For future clinical 
      application, trials using human BMCs would be indispensable. In this study, we 
      examined whether SF-1 could transform human BMCs into steroidogenic cells and 
      compared the steroid profile of these cells with that of mouse steroidogenic 
      BMCs. Primary cultured human BMCs infected with adenovirus containing bovine SF-1 
      cDNA could produce progesterone, corticosterone, cortisol, 
      dehydroepiandrosterone, testosterone, and estradiol. Such a mixed character of 
      adrenal and gonadal steroid production in human BMCs was supported by the 
      expressions of P450scc, 3beta-hydroxysteroid dehydrogenase (3beta-HSD), P450c21, 
      P450c11, P450c17, 17beta-HSD, and P450arom mRNAs. Unlike mouse steroidogenic 
      BMCs, introduction of SF-1 into human BMCs caused dramatic inductions of both 
      ACTH and LH receptors, thus leading to good responsiveness of the cells to ACTH 
      and LH respectively. Importantly, among several factors that are known to be 
      closely associated with adrenal and/or gonadal development, introduction of only 
      SF-1 enabled the human BMCs to express P450scc and to produce cortisol and 
      testosterone, suggesting that SF-1 is truly a master regulator for the production 
      of steroidogenic cells from human BMCs.
FAU - Tanaka, Tomoko
AU  - Tanaka T
AD  - Department of Medicine and Bioregulatory Science, Graduate School of Medical 
      Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
FAU - Gondo, Shigeki
AU  - Gondo S
FAU - Okabe, Taijiro
AU  - Okabe T
FAU - Ohe, Kenji
AU  - Ohe K
FAU - Shirohzu, Hisao
AU  - Shirohzu H
FAU - Morinaga, Hidetaka
AU  - Morinaga H
FAU - Nomura, Masatoshi
AU  - Nomura M
FAU - Tani, Kenzaburo
AU  - Tani K
FAU - Takayanagi, Ryoichi
AU  - Takayanagi R
FAU - Nawata, Hajime
AU  - Nawata H
FAU - Yanase, Toshihiko
AU  - Yanase T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers)
RN  - 0 (Receptors, Corticotropin)
RN  - 0 (Receptors, LH)
RN  - 0 (Steroidogenic Factor 1)
RN  - 0 (Steroids)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Adrenocorticotropic Hormone/metabolism
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/cytology/*physiology
MH  - Cattle
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Genetic Vectors/genetics/metabolism
MH  - Humans
MH  - Luteinizing Hormone/metabolism
MH  - Mesoderm/*cytology
MH  - Mice
MH  - Receptors, Corticotropin/genetics/metabolism
MH  - Receptors, LH/genetics/metabolism
MH  - Steroidogenic Factor 1/genetics/*metabolism
MH  - Steroids/*metabolism
EDAT- 2007/11/03 09:00
MHDA- 2008/03/25 09:00
CRDT- 2007/11/03 09:00
PHST- 2007/11/03 09:00 [pubmed]
PHST- 2008/03/25 09:00 [medline]
PHST- 2007/11/03 09:00 [entrez]
AID - 39/5/343 [pii]
AID - 10.1677/JME-07-0076 [doi]
PST - ppublish
SO  - J Mol Endocrinol. 2007 Nov;39(5):343-50. doi: 10.1677/JME-07-0076.