PMID- 17975841
OWN - NLM
STAT- MEDLINE
DCOM- 20080423
LR  - 20191210
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 86
IP  - 5
DP  - 2008 Apr
TI  - beta-Catenin and actin reorganization in HGF/SF response of ST14A cells.
PG  - 1044-52
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic factor that 
      activates proliferation, differentiation, and migration of various cell types. 
      Its action is mediated by c-Met, a receptor endowed with tyrosine kinase activity 
      that activates complex signaling cascades and mediates diverse cell responses. 
      Although HGF action was first demonstrated in epithelial cells, expression of HGF 
      and c-Met receptor has also been described in developing and adult mammalian 
      brain. In the developing central nervous system, areas of HGF and c-Met 
      expression are coincident with the migratory pathway of precursor cells. In the 
      present article we report that the interaction between c-Met and HGF/SF in 
      striatal progenitor ST14A cells triggers a signaling cascade that induces 
      modification of cell morphology, with decreased cell-cell interactions and 
      increased cell motility; in particular, we analyzed the reorganization of the 
      actin cytoskeleton and the delocalization of beta-catenin and N-cadherin. The 
      testing of other neurotrophic factors (NGF, BDNF, NT3, and CNTF) showed that the 
      observed modifications were peculiar to HGF. We show that phosphoinositide 
      3-kinase inhibitor treatment, which blocks cell scattering induced by HGF/SF, 
      does not abolish actin and beta-catenin redistribution. The effects of HGF/SF on 
      primary spinal cord cell cultures were also investigated, and HGF/SF was found to 
      have a possible motogenic effect on these cells. The data reported suggest that 
      HGF could play a role in the early steps of neurogenesis as a motogenic factor.
FAU - Soldati, C
AU  - Soldati C
AD  - Dipartimento di Biologia Cellulare e dello Sviluppo, Universita di Roma "La 
      Sapienza," Roma, Italy.
FAU - Biagioni, S
AU  - Biagioni S
FAU - Poiana, G
AU  - Poiana G
FAU - Augusti-Tocco, G
AU  - Augusti-Tocco G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Actins)
RN  - 0 (Cadherins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (beta Catenin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Actins/*metabolism
MH  - Animals
MH  - Cadherins/metabolism
MH  - Cell Adhesion/drug effects/physiology
MH  - Cell Differentiation/drug effects/physiology
MH  - Cell Line
MH  - Cell Movement/drug effects/physiology
MH  - Cells, Cultured
MH  - Central Nervous System/cytology/*embryology/metabolism
MH  - Cytoskeleton/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Hepatocyte Growth Factor/*metabolism/pharmacology
MH  - Mice
MH  - Neurons/drug effects/*metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Proto-Oncogene Proteins c-met/drug effects/metabolism
MH  - Rats
MH  - Stem Cells/drug effects/*metabolism
MH  - beta Catenin/*metabolism
EDAT- 2007/11/03 09:00
MHDA- 2008/04/24 09:00
CRDT- 2007/11/03 09:00
PHST- 2007/11/03 09:00 [pubmed]
PHST- 2008/04/24 09:00 [medline]
PHST- 2007/11/03 09:00 [entrez]
AID - 10.1002/jnr.21557 [doi]
PST - ppublish
SO  - J Neurosci Res. 2008 Apr;86(5):1044-52. doi: 10.1002/jnr.21557.