PMID- 17976680
OWN - NLM
STAT- MEDLINE
DCOM- 20080319
LR  - 20220316
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 371
IP  - 2
DP  - 2008 Feb 20
TI  - Attenuation of porcine reproductive and respiratory syndrome virus strain MN184 
      using chimeric construction with vaccine sequence.
PG  - 418-29
AB  - Two genetically distinct infectious recombinant virus clones (pMLV, constructed 
      from Ingelvac PRRS MLV and pMN184, constructed from virulent strain MN184) were 
      developed to study attenuation of contemporary porcine reproductive and 
      respiratory syndrome virus (PRRSV) strain MN184. Two reciprocal chimeric clones 
      (pMLVORF1/MN184 and pMN184ORF1/MLV) were then constructed, such that the 
      5'UTR/ORF1 of one genotype was linked to ORF2-7/3'UTR from the other genotype. In 
      vitro studies demonstrated that the rescued chimeric viruses possessed 
      intermediate growth properties compared to recombinant rMLV and rMN184. Swine 
      inoculation with rMN184 and rMLV verified that these viruses fully mimicked the 
      respective parent virus. In addition, earlier and higher antibody responses were 
      detected in animals infected with rMN184 in contrast to those infected with rMLV. 
      Chimeric virus treatment groups showed similar antibody responses as seen with 
      these parent viruses, but much less severe pathogenesis when compared to the 
      rMN184 group. These data suggested that genetic aspects of Ingelvac PRRS MLV 
      5'UTR/ORF1 replicase region and/or the structural proteins/3'UTR can serve to 
      attenuate virulent strain MN184. The data also indicated that designed PRRSV 
      vaccines could be developed, keeping the known 5'UTR/replicase region of an early 
      vaccine strain such as Ingelvac PRRS MLV intact, but replacing the structural 
      protein/3'UTR domain with that of an emerging virulent virus.
FAU - Wang, Yue
AU  - Wang Y
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint 
      Paul, MN 55108, USA.
FAU - Liang, Yajie
AU  - Liang Y
FAU - Han, Jun
AU  - Han J
FAU - Burkhart, Kelly M
AU  - Burkhart KM
FAU - Vaughn, Eric M
AU  - Vaughn EM
FAU - Roof, Michael B
AU  - Roof MB
FAU - Faaberg, Kay S
AU  - Faaberg KS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071031
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Complementary)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - 3' Untranslated Regions
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Open Reading Frames
MH  - Porcine Reproductive and Respiratory Syndrome/mortality/*pathology/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/immunology/metabolism/*pathogenicity
MH  - *Recombinant Fusion Proteins/genetics/metabolism
MH  - Swine
MH  - *Viral Vaccines
MH  - Virulence
EDAT- 2007/11/03 09:00
MHDA- 2008/03/20 09:00
CRDT- 2007/11/03 09:00
PHST- 2007/08/10 00:00 [received]
PHST- 2007/09/13 00:00 [revised]
PHST- 2007/09/21 00:00 [accepted]
PHST- 2007/11/03 09:00 [pubmed]
PHST- 2008/03/20 09:00 [medline]
PHST- 2007/11/03 09:00 [entrez]
AID - S0042-6822(07)00628-9 [pii]
AID - 10.1016/j.virol.2007.09.032 [doi]
PST - ppublish
SO  - Virology. 2008 Feb 20;371(2):418-29. doi: 10.1016/j.virol.2007.09.032. Epub 2007 
      Oct 31.