PMID- 17976910
OWN - NLM
STAT- MEDLINE
DCOM- 20080321
LR  - 20071123
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 429
IP  - 1
DP  - 2007 Dec 11
TI  - Peripheral lipopolysaccharide administration transiently affects expression of 
      brain-derived neurotrophic factor, corticotropin and proopiomelanocortin in mouse 
      brain.
PG  - 69-73
AB  - Peripheral inflammation induced by intraperitoneal (i.p.) injection of 
      Lipopolysaccharide (LPS) is known to cause functional impairments in the brain 
      affecting memory and learning. One of mechanisms may be the interference with 
      neurotrophin (NT) expression and function. In the current study we administered a 
      single, high dose of LPS (3mg/kg, i.p.) into mice and investigated changes in 
      brain-derived neurotrophic factor (BDNF) gene expression within 1-6 days after 
      LPS injection. Crude synaptosomes were isolated from brain tissue and subjected 
      to Western-blot analyses. We found transient reductions in synaptosomal proBDNF- 
      and BDNF protein expression, with a maximal decrease at day 3 as compared to 
      saline injected controls. The time course of reduction of BDNF mRNA in whole 
      brain extracts parallels the decrease in protein levels in synaptosomes. LPS 
      effects in the central nervous system (CNS) are known to crucially involve the 
      activation of the hypothalamic-pituitary-adrenal (HPA) axis. We analysed the time 
      course of corticotropin releasing hormone (CRH)- and proopiomelanocortin (POMC) 
      mRNA expression. As observed for BDNF-, CRH- and POMC mRNA levels are also 
      significantly reduced on day 3 indicating a comparable time course. These results 
      suggest that peripheral inflammation causes a reduction of trophic supply in the 
      brain, including BDNF at synaptic sites. The mechanisms involved could be a 
      negative feedback of the activated HPA axis.
FAU - Schnydrig, Sabine
AU  - Schnydrig S
AD  - Department of Physiology, University of Basel, Pestalozzistrasse 25, CH-4056 
      Basel, Switzerland.
FAU - Korner, Lukas
AU  - Korner L
FAU - Landweer, Svenja
AU  - Landweer S
FAU - Ernst, Beat
AU  - Ernst B
FAU - Walker, Gaby
AU  - Walker G
FAU - Otten, Uwe
AU  - Otten U
FAU - Kunz, Dieter
AU  - Kunz D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071011
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 66796-54-1 (Pro-Opiomelanocortin)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
SB  - IM
MH  - Adrenocorticotropic Hormone/genetics/*metabolism
MH  - Animals
MH  - Brain/*drug effects/metabolism
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Drug Administration Routes
MH  - Gene Expression/*drug effects
MH  - Lipopolysaccharides/*administration & dosage
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pro-Opiomelanocortin/genetics/*metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Time Factors
EDAT- 2007/11/03 09:00
MHDA- 2008/03/22 09:00
CRDT- 2007/11/03 09:00
PHST- 2007/06/18 00:00 [received]
PHST- 2007/09/24 00:00 [revised]
PHST- 2007/09/27 00:00 [accepted]
PHST- 2007/11/03 09:00 [pubmed]
PHST- 2008/03/22 09:00 [medline]
PHST- 2007/11/03 09:00 [entrez]
AID - S0304-3940(07)01076-2 [pii]
AID - 10.1016/j.neulet.2007.09.067 [doi]
PST - ppublish
SO  - Neurosci Lett. 2007 Dec 11;429(1):69-73. doi: 10.1016/j.neulet.2007.09.067. Epub 
      2007 Oct 11.