PMID- 17977213
OWN - NLM
STAT- MEDLINE
DCOM- 20071220
LR  - 20161021
IS  - 1110-4902 (Print)
VI  - 12
IP  - 2
DP  - 2005
TI  - Comparative study between cytotoxicity and flowcytometry crossmatches before and 
      after renal transplantation.
PG  - 77-89
AB  - Renal transplantation, in most countries, is based on human leukocyte antigen 
      (HLA) matching of the donor kidney with the recipient. Traditional human 
      leukocyte antigen matching is based on defining human leukocyte antigen 
      specificities by antibodies utilizing cytotoxicity crossmatch techniques. Newer 
      techniques have emerged, which challenge the accuracy of serological typing and 
      crossmatching. We compared the results of the standard complement-dependent 
      cytotoxicity crossmatch (CDCXM) with the anti-human globulin augmented 
      cytotoxicity (AHG-CDC), and Flowcytometry crossmatch (FCXM) for the detection of 
      anti-HLA antibodies in 150 pre-transplant patients. The development of 
      post-transplantation sensitization was screened utilizing these three techniques 
      within two weeks post-operative and correlated with rejection episodes. 
      Comparison between the results of CDCXM and AHG-CDC in 150 recipients, revealed 
      no significant correlation (P>0.05). When comparing these results with that of 
      FCXM in 50 recipients a significant correlation was shown (P<0.05). Relative to 
      CDCXM, the sensitivity of AHG-CDC was 100%, specificity 97.4%, positive 
      predictive value 92.3%, and negative predictive value 100%. On the other hand, 
      the sensitivity of FCXM was 100%, specificity 76.3%, positive predictive value 
      57.1%, and negative predictive value 100%. According to the results of CDCXM, 
      AHG-CDC, and FCXM, no difference was detected between pre- and posttransplant 
      anti-HLA sensitization within two weeks after the operation. Patients with 
      negative cytotoxicity crossmatch (CDCXM and AHG-CDC) and positive FCXM may have 
      an increased risk of early graft loss and may represent a relative 
      contraindication to transplantation. Given the important theoretical advantages 
      of FCXM over the CDC XM, further testing of the clinical relevance is warranted.
FAU - Abdel Rahman, Afaf S
AU  - Abdel Rahman AS
AD  - Department of Microbiology & Immunology, Faculty of Medicine, Ain Shams 
      University, Cairo, Egypt.
FAU - Fahim, Nehal M A
AU  - Fahim NM
FAU - El Sayed, Abeer A
AU  - El Sayed AA
FAU - El Hady, Soha A R
AU  - El Hady SA
FAU - Ahmad, Yasser S
AU  - Ahmad YS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Egypt
TA  - Egypt J Immunol
JT  - The Egyptian journal of immunology
JID - 9816016
RN  - 0 (HLA Antigens)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Adult
MH  - *Cytotoxicity Tests, Immunologic
MH  - Female
MH  - *Flow Cytometry
MH  - Graft Rejection/immunology
MH  - HLA Antigens/*immunology
MH  - *Histocompatibility Testing
MH  - Humans
MH  - Isoantibodies/*immunology/isolation & purification
MH  - Kidney Transplantation/*immunology
MH  - Male
MH  - Middle Aged
MH  - Sensitivity and Specificity
EDAT- 2007/11/06 09:00
MHDA- 2007/12/21 09:00
CRDT- 2007/11/06 09:00
PHST- 2007/11/06 09:00 [pubmed]
PHST- 2007/12/21 09:00 [medline]
PHST- 2007/11/06 09:00 [entrez]
PST - ppublish
SO  - Egypt J Immunol. 2005;12(2):77-89.