PMID- 17977619
OWN - NLM
STAT- MEDLINE
DCOM- 20080620
LR  - 20161124
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 52
IP  - 4-5
DP  - 2008 Mar-Apr
TI  - Comparative study of hydrogen peroxide- and 4-hydroxy-2-nonenal-induced cell 
      death in HT22 cells.
PG  - 776-85
AB  - Several studies have indicated that lipid peroxidation often occurs in response 
      to oxidative stress, and that many aldehydic products including 
      4-hydroxy-2-nonenal (HNE) are formed when lipid hydroperoxides break down. In 
      order to clarify the mechanism of oxidative stress-induced neuronal death in the 
      nervous system, we investigated H(2)O(2)- and HNE-induced cell death pathways in 
      HT22 cells, a mouse hippocampal cell line, under the same experimental 
      conditions. Treatment with H(2)O(2) and HNE decreased the viability of these 
      cells in a time- and concentration-dependent manner. In the cells treated with 
      H(2)O(2), significant increases in the immunoreactivities of DJ-1 and nuclear 
      factor-kappaB (NF-kappaB) subunits (p65 and p50) were observed in the nuclear 
      fraction. H(2)O(2) also induced an increase in the intracellular concentration of 
      Ca(2+), and cobalt chloride (CoCl(2)), a Ca(2+) channel inhibitor, suppressed the 
      H(2)O(2)-induced cell death. In HNE-treated cells, none of these phenomena were 
      observed; however, HNE adduct proteins were formed after exposure to HNE, but not 
      to H(2)O(2). N-Acetyl-L-cysteine (NAC) suppressed both HNE-induced cell death and 
      HNE-induced expression of HNE adduct proteins, whereas H(2)O(2)-induced cell 
      death was not affected. These findings suggest that the mechanisms of cell death 
      induced by H(2)O(2) different from those induced by HNE in HT22 cells, and that 
      HNE adduct proteins play an important role in HNE-induced cell death. It is also 
      suggested that the pathway for H(2)O(2)-induced cell death in HT22 cells does not 
      involve HNE production.
FAU - Ishimura, Atsushi
AU  - Ishimura A
AD  - Research Unit of Pharmacology, Department of Clinical Pharmacy, College of 
      Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, 
      Japan.
FAU - Ishige, Kumiko
AU  - Ishige K
FAU - Taira, Takahiro
AU  - Taira T
FAU - Shimba, Shigeki
AU  - Shimba S
FAU - Ono, Shin-Ichi
AU  - Ono S
FAU - Ariga, Hiroyoshi
AU  - Ariga H
FAU - Tezuka, Masakatsu
AU  - Tezuka M
FAU - Ito, Yoshihisa
AU  - Ito Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070916
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Aldehydes)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (NF-kappa B)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Oxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 36015-30-2 (Propidium)
RN  - 3G0H8C9362 (Cobalt)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EUY85H477I (thiazolyl blue)
RN  - EVS87XF13W (cobaltous chloride)
RN  - K1CVM13F96 (4-hydroxy-2-nonenal)
RN  - SY7Q814VUP (Calcium)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Aldehydes/*toxicity
MH  - Blotting, Western
MH  - Calcium/metabolism
MH  - Cell Death/*drug effects
MH  - Cell Line, Tumor
MH  - Cobalt/pharmacology
MH  - Flow Cytometry
MH  - Free Radical Scavengers/pharmacology
MH  - Humans
MH  - Hydrogen Peroxide/*toxicity
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Lipid Peroxidation/drug effects
MH  - NF-kappa B/physiology
MH  - Neurons/drug effects
MH  - Neuroprotective Agents/pharmacology
MH  - Oxidants/*toxicity
MH  - Oxidative Stress/drug effects
MH  - Propidium
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/drug effects
MH  - Tetrazolium Salts
MH  - Thiazoles
EDAT- 2007/11/06 09:00
MHDA- 2008/06/21 09:00
CRDT- 2007/11/06 09:00
PHST- 2007/05/31 00:00 [received]
PHST- 2007/08/22 00:00 [revised]
PHST- 2007/09/13 00:00 [accepted]
PHST- 2007/11/06 09:00 [pubmed]
PHST- 2008/06/21 09:00 [medline]
PHST- 2007/11/06 09:00 [entrez]
AID - S0197-0186(07)00261-6 [pii]
AID - 10.1016/j.neuint.2007.09.008 [doi]
PST - ppublish
SO  - Neurochem Int. 2008 Mar-Apr;52(4-5):776-85. doi: 10.1016/j.neuint.2007.09.008. 
      Epub 2007 Sep 16.