PMID- 17979221
OWN - NLM
STAT- MEDLINE
DCOM- 20080108
LR  - 20071116
IS  - 0951-4198 (Print)
IS  - 0951-4198 (Linking)
VI  - 21
IP  - 23
DP  - 2007
TI  - Screening and confirmation of 100 pesticides in food samples by liquid 
      chromatography/tandem mass spectrometry.
PG  - 3869-82
AB  - An analytical method for screening, quantitation and confirmation of a group of 
      100 pesticides in vegetable and fruit samples was developed using liquid 
      chromatography coupled with tandem mass spectrometry (LC/MS/MS). The pesticides 
      studied belonged to different chemical families of herbicides, insecticides and 
      fungicides; some degradation products were included as well. A thorough 
      optimization was performed for each analyte to achieve individual optimum 
      fragmentor and collision energy voltages. Two transitions per parent compound 
      were monitored in a single chromatographic run containing two time segments. A 
      small particle size C(18) column (1.8 microm) was used for the chromatographic 
      separation of the mixture, providing very narrow peaks and allowing an excellent 
      separation of all the analytes in a 30-min period for maximum peak capacity. The 
      method was validated with blank matrices of green pepper, tomato and orange 
      spiked from 0.1 to 100 microg/kg with the pesticide mix. Quantitation was carried 
      out using matrix-matched standard calibration and linearity of response over 3 
      orders of magnitude was demonstrated (r > 0.99). Limits of detection based on two 
      transitions and ion-ratio requirements ranged between 0.3 and 50 microg/kg. In 
      general, the sensitivity obtained meets the maximum residue levels (MRLs) 
      established by the European Union regulation for food monitoring programs. The 
      analytical performance of the method was evaluated for different types of 
      vegetables and fruits, showing little or no matrix effects, and examples of 
      screening and confirmation of pesticides in these samples are shown here.
CI  - Copyright 2007 John Wiley & Sons, Ltd.
FAU - Ferrer, Imma
AU  - Ferrer I
AD  - Pesticide Residue Research Group, University of Almeria, Almeria, Spain. 
      iferrer@ual.es
FAU - Thurman, E Michael
AU  - Thurman EM
FAU - Zweigenbaum, Jerry A
AU  - Zweigenbaum JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Pesticides)
SB  - IM
MH  - Chromatography, High Pressure Liquid/methods
MH  - Food Analysis/*methods
MH  - Food Contamination/*analysis
MH  - Pesticides/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2007/11/06 09:00
MHDA- 2008/01/09 09:00
CRDT- 2007/11/06 09:00
PHST- 2007/11/06 09:00 [pubmed]
PHST- 2008/01/09 09:00 [medline]
PHST- 2007/11/06 09:00 [entrez]
AID - 10.1002/rcm.3268 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2007;21(23):3869-82. doi: 10.1002/rcm.3268.