PMID- 17979816
OWN - NLM
STAT- MEDLINE
DCOM- 20080103
LR  - 20190911
IS  - 0929-8665 (Print)
IS  - 0929-8665 (Linking)
VI  - 14
IP  - 8
DP  - 2007
TI  - Structural characterization of eight cyclic lipopeptides produced by Bacillus 
      subtilis HSO121.
PG  - 766-73
AB  - Lipopeptides are amphiphilic compounds which contain both hydrophobic fatty acid 
      moieties and amphiphilic peptide moieties. From the cell-free broth of Bacillus 
      subtilis HSO121, eight cyclic lipopeptides were isolated by reversed-phase high 
      performance liquid chromatography (RP-HPLC). The peptide part of each lipopeptide 
      was elucidated according to electrospray ionization quadruple-time-of-flight mass 
      spectrometry (ESI Q-TOF MS) and the fatty acid part was analyzed by 
      electroionization gas chromatography/mass spectrometry (EI GC/MS). It showed that 
      fractions 1-8 had molecular masses of 1007, 1021, 1021, 1035, 1035, 1035, 1063, 
      and 1049, respectively. Analysis of hydrolyzed lipopeptides revealed that they 
      had invariant amino acid compositions. The differences in molecular weights 
      represent changes in the number of methylene groups and different types of 
      branched chains in fatty acids. Peptide sequences of two of the eight 
      lipopeptides appeared to be N-Asp-Leu-Leu-Val-Glu-Leu-Leu-C, which was different 
      from previously reported lipopeptides. The remaining six had an identical peptide 
      sequence of N-Glu-Leu-Leu-Val-Asp-Leu-Leu-C. The fatty acid parts were found to 
      be mixtures of iso C(12), iso C(13), anteiso C(13), iso C(14), n C(14), iso 
      C(15), anteiso C(15), n C(15), anteiso C(16) and anteiso C(17) beta-hydroxy fatty 
      acids. The structure of each lipopeptide was determined to be the beta-hydroxy 
      fatty acid bonded to the peptide chain.
FAU - Liu, Xiangyang
AU  - Liu X
AD  - Laboratory for Advanced Materials and Institute of Applied Chemistry, East China 
      University of Science and Technology, Shanghai, China.
FAU - Haddad, Namir I A
AU  - Haddad NI
FAU - Yang, Shizhong
AU  - Yang S
FAU - Mu, Bozhong
AU  - Mu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Protein Pept Lett
JT  - Protein and peptide letters
JID - 9441434
RN  - 0 (Fatty Acids)
RN  - 0 (Lipoproteins)
RN  - 0 (Peptides, Cyclic)
SB  - IM
MH  - Bacillus subtilis/*metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Fatty Acids/chemistry
MH  - Lipoproteins/biosynthesis/*chemistry
MH  - Peptides, Cyclic/biosynthesis/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
EDAT- 2007/11/06 09:00
MHDA- 2008/01/04 09:00
CRDT- 2007/11/06 09:00
PHST- 2007/11/06 09:00 [pubmed]
PHST- 2008/01/04 09:00 [medline]
PHST- 2007/11/06 09:00 [entrez]
AID - 10.2174/092986607781483642 [doi]
PST - ppublish
SO  - Protein Pept Lett. 2007;14(8):766-73. doi: 10.2174/092986607781483642.