PMID- 17980465
OWN - NLM
STAT- MEDLINE
DCOM- 20080225
LR  - 20081121
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 25
IP  - 49
DP  - 2007 Nov 28
TI  - Construction and immunogenicity of pseudotype baculovirus expressing GP5 and M 
      protein of porcine reproductive and respiratory syndrome virus.
PG  - 8220-7
AB  - Baculovirus, which is extensively utilized as an excellent tool for production of 
      recombinant protein in insect cells, has recently emerged as a novel and 
      attractive gene delivery vehicle for mammalian cells. In the present study, a 
      pseudotype baculovirus (with the glycoprotein of vesicular stomatitis virus 
      (VSV-G) on the envelope) was used as vector to construct recombinant baculovirus 
      coexpressing GP5 and M protein of porcine reproductive and respiratory syndrome 
      virus (PRRSV), under the transcriptional control of two independent 
      cytomegalovirus immediate early (CMV-IE) enhancer/promoters. The resultant 
      recombinant baculovirus (BV-G-5m6) efficiently expressed PRRSV GP5 and M protein 
      in mammalian cells. Intramuscular injection of BV-G-5m6 with various doses (1 x 
      10(8), 1 x 10(9), and 1 x 10(10)PFU/mouse) induced the production of 
      PRRSV-specific neutralizing antibodies and gamma interferon (IFN-gamma) under 
      dose-dependent pattern. Furthermore, BV-G-5m6 performed better immunogenicity, 
      even at low dose (10(8)PFU), than DNA construct (pCI-5m6) encoding the same 
      antigens, as demonstrated by significantly enhanced neutralizing antibodies and 
      IFN-gamma production. These results indicate that pseudotype baculovirus-mediated 
      gene delivery can be utilized as an alternative strategy to develop new 
      generation of vaccine against PRRSV infection.
FAU - Wang, Shengping
AU  - Wang S
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan 430070, Hubei, P.R.China.
FAU - Fang, Liurong
AU  - Fang L
FAU - Fan, Huiying
AU  - Fan H
FAU - Jiang, Yunbo
AU  - Jiang Y
FAU - Pan, Yongfei
AU  - Pan Y
FAU - Luo, Rui
AU  - Luo R
FAU - Zhao, Qian
AU  - Zhao Q
FAU - Chen, Huanchun
AU  - Chen H
FAU - Xiao, Shaobo
AU  - Xiao S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071022
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Viral)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Baculoviridae/*genetics/immunology/metabolism
MH  - Cell Line
MH  - Female
MH  - Interferon-gamma/biosynthesis
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neutralization Tests
MH  - Porcine Reproductive and Respiratory Syndrome/prevention & control
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/immunology/metabolism
MH  - *Recombination, Genetic
MH  - Swine
MH  - *Viral Envelope Proteins/genetics/immunology/metabolism
MH  - *Viral Matrix Proteins/genetics/immunology/metabolism
MH  - Viral Vaccines/administration & dosage/genetics/immunology
EDAT- 2007/11/06 09:00
MHDA- 2008/02/26 09:00
CRDT- 2007/11/06 09:00
PHST- 2007/07/03 00:00 [received]
PHST- 2007/09/19 00:00 [revised]
PHST- 2007/09/25 00:00 [accepted]
PHST- 2007/11/06 09:00 [pubmed]
PHST- 2008/02/26 09:00 [medline]
PHST- 2007/11/06 09:00 [entrez]
AID - S0264-410X(07)01106-1 [pii]
AID - 10.1016/j.vaccine.2007.09.069 [doi]
PST - ppublish
SO  - Vaccine. 2007 Nov 28;25(49):8220-7. doi: 10.1016/j.vaccine.2007.09.069. Epub 2007 
      Oct 22.