PMID- 17980908
OWN - NLM
STAT- MEDLINE
DCOM- 20080414
LR  - 20131121
IS  - 0143-4179 (Print)
IS  - 0143-4179 (Linking)
VI  - 41
IP  - 6
DP  - 2007 Dec
TI  - Roles of the bed nucleus of stria terminalis and of the amygdala in 
      N/OFQ-mediated anxiety and HPA axis activation.
PG  - 399-410
AB  - Nociceptin/orphanin FQ (N/OFQ) is an opioid-related neuropeptide that is widely 
      distributed in limbic regions of the brain. After intracerebroventricular (icv) 
      injections in rodents, N/OFQ produces elevations in 
      hypothalamic-pituitary-adrenal (HPA) axis activity, and has been reported to 
      produce both anxiogenic and anxiolytic actions. We examined the neuroanatomical 
      basis of these effects with injections of N/OFQ (0.01-1.0nmol) into the lateral 
      ventricle, the amygdala, and the bed nucleus of stria terminalis (BNST) in 
      independent groups of well-handled rats under low stress conditions. 
      Anxiety-related behaviors were evaluated in a neophobic test of anxiety. The 
      latency to enter, total time spent in, and number of entries into an unfamiliar 
      open field and its central zone were measured. After the open field testing, 
      plasma samples were obtained for analysis of HPA axis activity. The N/OFQ-treated 
      rats displayed more anxiety-related behaviors than vehicle-treated rats did with 
      all three of the injection types. However, these effects were greater and more 
      consistent after the icv injections (0.01-1.0nmol) than they were after the 
      amygdala (0.10-1.0nmol) or BNST (1.0nmol) injections. The icv and BNST injections 
      also produced elevations in circulating corticosterone, indicating that the HPA 
      axis was activated in these rats. Intra-amygdaloid injections did not affect 
      corticosterone levels during the open field testing. These results indicate that 
      the amygdala and BNST participate in the anxiogenic behavioral effects of N/OFQ. 
      However, since the most potent effects were seen after icv N/OFQ injections, the 
      anxiogenic and HPA axis-activating effects of N/OFQ appear to occur through 
      additive actions in multiple limbic (and perhaps cortical and brainstem) sites.
FAU - Green, Megan K
AU  - Green MK
AD  - University of Florida, Department of Psychology, Behavioral Neuroscience Program, 
      P.O. Box 112250, Gainesville, FL 32611-2250, USA.
FAU - Barbieri, Emily V
AU  - Barbieri EV
FAU - Brown, Brandon D
AU  - Brown BD
FAU - Chen, Kir-Wei
AU  - Chen KW
FAU - Devine, Darragh P
AU  - Devine DP
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20071105
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (Opioid Peptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (nociceptin orphanin FQ(1-17)OH)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Amygdala/*physiology
MH  - Animals
MH  - Anxiety/chemically induced/*physiopathology
MH  - Behavior, Animal
MH  - Corticosterone/analysis
MH  - Hypothalamo-Hypophyseal System/drug effects/*metabolism
MH  - Limbic System/*physiology
MH  - Male
MH  - Opioid Peptides/administration & dosage/*pharmacology
MH  - Peptide Fragments/administration & dosage/*pharmacology
MH  - Pituitary-Adrenal System/drug effects/*metabolism
MH  - Rats
MH  - Rats, Long-Evans
EDAT- 2007/11/06 09:00
MHDA- 2008/04/15 09:00
CRDT- 2007/11/06 09:00
PHST- 2007/05/24 00:00 [received]
PHST- 2007/09/09 00:00 [revised]
PHST- 2007/09/12 00:00 [accepted]
PHST- 2007/11/06 09:00 [pubmed]
PHST- 2008/04/15 09:00 [medline]
PHST- 2007/11/06 09:00 [entrez]
AID - S0143-4179(07)00087-X [pii]
AID - 10.1016/j.npep.2007.09.002 [doi]
PST - ppublish
SO  - Neuropeptides. 2007 Dec;41(6):399-410. doi: 10.1016/j.npep.2007.09.002. Epub 2007 
      Nov 5.