PMID- 17980966
OWN - NLM
STAT- MEDLINE
DCOM- 20080321
LR  - 20211203
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 429
IP  - 1
DP  - 2007 Dec 11
TI  - Insulin rescues ES cell-derived neural progenitor cells from apoptosis by 
      differential regulation of Akt and ERK pathways.
PG  - 49-54
AB  - Transplantation of embryonic stem (ES) cell-derived neural progenitor cells 
      (ES-NPCs) is one promising technology for the treatment of spinal cord injury. 
      Promoting ES-NPC survival at the lesion site is critical for the successful 
      treatment. We tested the role of insulin in promoting mouse ES-NPC survival. 
      Cultured ES-NPCs survived when maintained in normoxia but underwent apoptosis 
      when exposed to hypoxia. Insulin rescued ES-NPCs from hypoxia-induced cell death. 
      This effect could be blocked by the phosphatidylinositol 3-kinase (PI3K)/Akt 
      pathway inhibitor LY294002. In contrast, mitogen-activated protein kinase 
      (MAP)/extracellular-signal-regulated kinase (ERK) pathway inhibitor U0126 
      potentiated insulin-mediated survival. Immunoblots revealed that insulin 
      upregulated activation of Akt and inhibited ERK activation through the PI3K 
      pathway. In addition, we showed that insulin reduced the activation of caspase-3, 
      the key executor of apoptosis. In summary, our data suggest that insulin prevent 
      apoptosis in ES-NPCs by activating Akt and inhibiting ERK through the PI3K 
      pathway.
FAU - Zhao, Yanhong
AU  - Zhao Y
AD  - Key Laboratory of Molecular Developmental Biology, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, 
      Beijing 100080, People's Republic of China.
FAU - Xiao, Zhifeng
AU  - Xiao Z
FAU - Gao, Yuan
AU  - Gao Y
FAU - Chen, Bing
AU  - Chen B
FAU - Zhao, Yannan
AU  - Zhao Y
FAU - Zhang, Jing
AU  - Zhang J
FAU - Dai, Jianwu
AU  - Dai J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071012
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Butadienes)
RN  - 0 (Chromones)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (Morpholines)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nitriles)
RN  - 0 (U 0126)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Butadienes/pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Chromones/pharmacology
MH  - Drug Interactions
MH  - Embryo, Mammalian
MH  - Embryonic Stem Cells
MH  - Enzyme Inhibitors/pharmacology
MH  - Extracellular Signal-Regulated MAP Kinases
MH  - Gene Expression Regulation/drug effects
MH  - Hypoglycemic Agents/*pharmacology
MH  - Hypoxia/drug therapy/physiopathology
MH  - Insulin/*pharmacology
MH  - Mice
MH  - Morpholines/pharmacology
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/*drug effects
MH  - Nitriles/pharmacology
MH  - Oncogene Protein v-akt
MH  - Protein Serine-Threonine Kinases/*physiology
MH  - Signal Transduction/drug effects
EDAT- 2007/11/06 09:00
MHDA- 2008/03/22 09:00
CRDT- 2007/11/06 09:00
PHST- 2007/08/09 00:00 [received]
PHST- 2007/09/28 00:00 [revised]
PHST- 2007/09/30 00:00 [accepted]
PHST- 2007/11/06 09:00 [pubmed]
PHST- 2008/03/22 09:00 [medline]
PHST- 2007/11/06 09:00 [entrez]
AID - S0304-3940(07)01071-3 [pii]
AID - 10.1016/j.neulet.2007.09.076 [doi]
PST - ppublish
SO  - Neurosci Lett. 2007 Dec 11;429(1):49-54. doi: 10.1016/j.neulet.2007.09.076. Epub 
      2007 Oct 12.