PMID- 17982620
OWN - NLM
STAT- MEDLINE
DCOM- 20080123
LR  - 20071105
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 18
IP  - 6
DP  - 2007 Dec
TI  - A combined strategy of conventional cytogenetics, fluorescent in situ 
      hybridization and microsatellite polymerase chain reaction to analyze the 
      deletion of chromosome 6 in laryngeal squamous cell carcinoma.
PG  - 1379-85
AB  - Laryngeal squamous cell carcinoma (LSCC) is a common cancer of the upper 
      respiratory tract. The cytogenetic and molecular events involved in the 
      pathogenesis of LSCC are not well understood. In this study, a combined strategy 
      of conventional cytogenetics, fluorescent in situ hybridization (FISH) and 
      microsatellite polymerase chain reaction was performed to analyze the deletion of 
      chromosome 6 in LSCC. Karyotype analysis indicated that the deletions of 6q were 
      marker chromosomes potentially specific to LSCC. To further characterize the loss 
      of 6q, metaphase cells derived from both solid tumors and the Hep-2 cell line 
      were investigated using FISH, with results consistent with those of conventional 
      cytogenetics. Moreover, tumor-adjacent normal tissue DNA pairs from 70 LSCC in 
      China were analyzed for loss of heterozygosity (LOH) and microsatellite 
      instability (MI) on chromosome 6q in the region 6q25 by polymerase chain reaction 
      (PCR) and three microsatellite markers. Overall, the highest frequency of 
      microsatellite alteration (68.2%) was located at D6S980, which revealed that the 
      region around D6S980 in 6q25 might harbor some important genes related to the 
      pathogenesis of LSCC in Chinese patients. The pattern of chromosomal changes 
      detected using the combined strategy suggests that it will become the most 
      suitable way to find novel cancer-related genes and discover the relationship 
      between the aberration of genetics and their tumorigenic mechanism.
FAU - Kang, Ning
AU  - Kang N
AD  - Department of Medical Genetics, China Medical University, Shenyang 110001, PR 
      China.
FAU - Li, Fucai
AU  - Li F
FAU - Fu, Weineng
AU  - Fu W
FAU - Zhang, Jinghai
AU  - Zhang J
FAU - Sun, Kailai
AU  - Sun K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Differentiation
MH  - *Chromosome Deletion
MH  - Chromosome Painting
MH  - *Chromosomes, Human, Pair 6
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - *Microsatellite Repeats
MH  - Neoplasm Staging
MH  - Polymerase Chain Reaction/methods
EDAT- 2007/11/06 09:00
MHDA- 2008/01/24 09:00
CRDT- 2007/11/06 09:00
PHST- 2007/11/06 09:00 [pubmed]
PHST- 2008/01/24 09:00 [medline]
PHST- 2007/11/06 09:00 [entrez]
PST - ppublish
SO  - Oncol Rep. 2007 Dec;18(6):1379-85.