PMID- 17983376
OWN - NLM
STAT- MEDLINE
DCOM- 20080122
LR  - 20191210
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 62
IP  - 12
DP  - 2007 Dec
TI  - A loose-fit coculture of activated keratinocytes and dendritic cell-related cells 
      for prediction of sensitizing potential.
PG  - 1419-28
AB  - Protection against contact allergy begins with the collection of reliable data 
      about the sensitizing potential of chemicals. Today, the local lymph node assay 
      (LLNA) in mice is widely used to identify sensitizing substances. For several 
      reasons, an in vitro assay could be preferable to animal experiments. We propose 
      an in vitro test for the detection of a sensitizing potential of a chemical 
      composed of a single layer of human nondifferentiating keratinocytes and of 
      allogenic floating monocytes which are cocultured in serum-free medium in the 
      presence of a cytokine cocktail. Within days, the coculture develops to an 
      allergen- sensitive system consisting of activated keratinocytes and of mobile 
      dendritic cell-related cells (DC-related cell). The sensitizing potential can be 
      determined by analyzing the expression of the dendritic cell maturation marker 
      CD86. For the model contact allergens tested so far [trinitrobenzenesulfonic acid 
      (TNBS), phenylendiamine, and 4-aminoacetanilide], the strength of the reaction 
      was in concordance with results from the LLNA. Sensitivity of the assay allowed 
      testing at concentrations without general cytotoxicity. Thus, a differentiation 
      between allergens and irritants was possible. Regarding cytokine secretion, the 
      assay distinguished between the allergen TNBS and the Toll-like receptor ligand 
      lipopolysaccharide. The coculture can be set up from cryopreserved cells. The 
      assay is easy to perform and reproducible. Donor-variance is negligible. This in 
      vitro assay based on a loose-fit coculture is a reasonable approach to screen for 
      the sensitizing potential of xenobiotics and might partially replace the LLNA and 
      other animal tests.
FAU - Schreiner, M
AU  - Schreiner M
AD  - Institute of Molecular Biology and Bioinformatics, Charite, Berlin, Germany.
FAU - Peiser, M
AU  - Peiser M
FAU - Briechle, D
AU  - Briechle D
FAU - Stahlmann, R
AU  - Stahlmann R
FAU - Zuberbier, T
AU  - Zuberbier T
FAU - Wanner, R
AU  - Wanner R
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Phenylenediamines)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
RN  - U770QIT64J (4-phenylenediamine)
SB  - IM
MH  - Allergens/*immunology
MH  - B7-2 Antigen/*metabolism
MH  - Cell Differentiation
MH  - Coculture Techniques/methods
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Dermatitis, Allergic Contact/*etiology
MH  - Flow Cytometry
MH  - Humans
MH  - Keratinocytes/cytology/drug effects/*immunology
MH  - Local Lymph Node Assay
MH  - Monocytes/chemistry/cytology/drug effects/immunology
MH  - Phenylenediamines/*immunology/toxicity
MH  - Skin Tests/*methods
MH  - Trinitrobenzenesulfonic Acid/*immunology/toxicity
EDAT- 2007/11/07 09:00
MHDA- 2008/01/23 09:00
CRDT- 2007/11/07 09:00
PHST- 2007/11/07 09:00 [pubmed]
PHST- 2008/01/23 09:00 [medline]
PHST- 2007/11/07 09:00 [entrez]
AID - ALL1511 [pii]
AID - 10.1111/j.1398-9995.2007.01511.x [doi]
PST - ppublish
SO  - Allergy. 2007 Dec;62(12):1419-28. doi: 10.1111/j.1398-9995.2007.01511.x.