PMID- 17983593
OWN - NLM
STAT- MEDLINE
DCOM- 20080118
LR  - 20071206
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 365
IP  - 3
DP  - 2008 Jan 18
TI  - Expression of vascular cell adhesion molecule-1 indicates the differentiation 
      potential of human bone marrow stromal cells.
PG  - 406-12
AB  - Bone marrow stromal cells (BMSCs) are a mixture of cells differing in 
      differentiation potential including mesenchymal stem cells, and so far no CD 
      antigens were found to be predictable for the differentiation property of each 
      BMSC. Here we attempted to isolate differentiation-associated CD antigens using 
      100 immortalized human BMSC (ihBMSC) clones. Among 13 CD antigens analyzed, only 
      CD106/Vascular cell adhesion molecule-1 (VCAM-1) showed a clear correlation with 
      the differentiation potential of each clone; CD106-positive ihBMSC clones were 
      less osteogenic and more adipogenic than CD106-negative clones. This association 
      was confirmed in primary BMSCs sorted by CD106, showing that the CD106-positive 
      fraction contained less osteogenic and more adipogenic cells than the 
      CD106-positive fraction. The evaluation of CD106 fraction of BMSC strains in 
      early passages predicted clearly the osteogenic and adipogenic potential after in 
      vitro induction of differentiation, indicating the usefulness of CD106 as a 
      differentiation-predicting marker of BMSC.
FAU - Fukiage, Kenichi
AU  - Fukiage K
AD  - Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto 
      University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
FAU - Aoyama, Tomoki
AU  - Aoyama T
FAU - Shibata, Kotaro R
AU  - Shibata KR
FAU - Otsuka, Seiji
AU  - Otsuka S
FAU - Furu, Moritoshi
AU  - Furu M
FAU - Kohno, Yoshiki
AU  - Kohno Y
FAU - Ito, Kinya
AU  - Ito K
FAU - Jin, Yonghui
AU  - Jin Y
FAU - Fujita, Satoshi
AU  - Fujita S
FAU - Fujibayashi, Shunsuke
AU  - Fujibayashi S
FAU - Neo, Masashi
AU  - Neo M
FAU - Nakayama, Tomitaka
AU  - Nakayama T
FAU - Nakamura, Takashi
AU  - Nakamura T
FAU - Toguchida, Junya
AU  - Toguchida J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071105
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, CD)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
SB  - IM
MH  - *Adipogenesis
MH  - Antigens, CD/analysis/metabolism
MH  - Bone Marrow Cells/chemistry/*cytology/metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Humans
MH  - *Osteogenesis
MH  - Stromal Cells/chemistry/cytology/metabolism
MH  - Vascular Cell Adhesion Molecule-1/analysis/*metabolism
EDAT- 2007/11/07 09:00
MHDA- 2008/01/19 09:00
CRDT- 2007/11/07 09:00
PHST- 2007/10/11 00:00 [received]
PHST- 2007/10/22 00:00 [accepted]
PHST- 2007/11/07 09:00 [pubmed]
PHST- 2008/01/19 09:00 [medline]
PHST- 2007/11/07 09:00 [entrez]
AID - S0006-291X(07)02293-0 [pii]
AID - 10.1016/j.bbrc.2007.10.149 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2008 Jan 18;365(3):406-12. doi: 
      10.1016/j.bbrc.2007.10.149. Epub 2007 Nov 5.