PMID- 17984043
OWN - NLM
STAT- MEDLINE
DCOM- 20080115
LR  - 20221207
IS  - 1080-2924 (Print)
IS  - 1080-2924 (Linking)
VI  - 13
IP  - 3
DP  - 2007
TI  - Clinical significance of neutrophil apoptosis in peripheral blood of patients 
      with type 2 diabetes mellitus.
PG  - 108-12
AB  - Although neutrophils are essential components of the natural immune system, they 
      have also been implicated in the pathogenesis of tissue injuries. We assessed the 
      clinical significance of neutrophil apoptosis in the peripheral blood of patients 
      with type 2 diabetes mellitus (T2DM). PATIENTS AND METHODS: The study included 52 
      patients with T2DM (30 men, 22 women). Control subjects were 16 healthy 
      volunteers without diabetes (7 men, 9 women). Neutrophil apoptosis levels were 
      measured active caspase-3 positive rate by flow cytometry. RESULTS: The mean rate 
      of neutrophil apoptosis in patients with T2DM was 15.0% (95% confidence interval 
      [CI]: 9.5% approximately 20.5%), while that in the control group was 5.8% (95% 
      CI: 1.6% approximately 10.0%). There were significant negative correlations 
      between neutrophil apoptosis rate and hemoglobin (Hb) A1c levels (r = -0.352, P < 
      .01). The mean rate of neutrophil apoptosis in the patient group with the 3 major 
      complications (diabetic retinopathy, nephropathy, and neuropathy) was 11.1% (95% 
      CI: 5.5%-16.7%, n = 36) and that of another group without complications was 23.8% 
      (95% CI: 11.4%-36.2%, n = 16). There was a significant difference between these 2 
      groups (P < .05). CONCLUSIONS: The neutrophil apoptosis rate in patients with 
      T2DM was significantly correlated with HbA1C levels. The mean rate of neutrophil 
      apoptosis in the patient group with 3 major diabetic complications remained lower 
      than that in another patient group without complications. The inhibition of 
      neutrophil apoptosis by chronic hyperglycemia is thought to promote tissue injury 
      and to enhance the risk of microangiopathy.
FAU - Sudo, Chiaki
AU  - Sudo C
AD  - Department of Laboratory Sciences, Gunma University School of Health Sciences, 
      Japan.
FAU - Ogawara, Hatsue
AU  - Ogawara H
FAU - Saleh, Almaqoul Wedad Hamdi
AU  - Saleh AW
FAU - Nishimoto, Natsumi
AU  - Nishimoto N
FAU - Utsugi, Toshihiro
AU  - Utsugi T
FAU - Ooyama, Yoshio
AU  - Ooyama Y
FAU - Fukumura, Yukihito
AU  - Fukumura Y
FAU - Murakami, Masami
AU  - Murakami M
FAU - Handa, Hiroshi
AU  - Handa H
FAU - Tomono, Shoichi
AU  - Tomono S
FAU - Murakami, Hirokazu
AU  - Murakami H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Lab Hematol
JT  - Laboratory hematology : official publication of the International Society for 
      Laboratory Hematology
JID - 9615688
RN  - 0 (Glycated Hemoglobin A)
SB  - IM
MH  - Aged
MH  - *Apoptosis
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/*blood
MH  - Diabetic Nephropathies/blood
MH  - Diabetic Neuropathies/blood
MH  - Diabetic Retinopathy/blood
MH  - Female
MH  - Glycated Hemoglobin/agonists
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Neutrophils/*pathology
EDAT- 2007/11/07 09:00
MHDA- 2008/01/16 09:00
CRDT- 2007/11/07 09:00
PHST- 2007/11/07 09:00 [pubmed]
PHST- 2008/01/16 09:00 [medline]
PHST- 2007/11/07 09:00 [entrez]
AID - J81L674327604117 [pii]
AID - 10.1532/LH96.07003 [doi]
PST - ppublish
SO  - Lab Hematol. 2007;13(3):108-12. doi: 10.1532/LH96.07003.