PMID- 17985234
OWN - NLM
STAT- MEDLINE
DCOM- 20080603
LR  - 20211203
IS  - 0364-3190 (Print)
IS  - 0364-3190 (Linking)
VI  - 33
IP  - 4
DP  - 2008 Apr
TI  - Dex-ras1 and serum- and glucocorticoid-inducible protein kinase 1: regulation of 
      expression by dexamethasone in HEK293 cells.
PG  - 609-13
AB  - The molecular and cellular basis of the psychotropic actions of adrenal 
      corticosteroids is poorly understood. Previously, we reported that modulation of 
      large conductance Ca2+-activated potassium channel (BK-channel) function by 
      glucocorticoids can be recapitulated in human embryonic kidney293 (HEK293) cells 
      (J Physiol 537:57, 2001). In the present paper, we examined the effect of 
      dexamethasone on the expression of candidate mediator proteins of glucocorticoid 
      action, dex-ras1 and serum and glucocorticoid inducible protein kinase 1 (SGK), 
      in HEK293 cells. Dex-ras1 mRNA was readily detectable under basal conditions 
      however, no changes of dex-ras1 mRNA expression occurred upon exposure to 100 nM 
      of dexamethasone for 2 h. In contrast, a 2.5-fold increase of SGK mRNA was found 
      under similar conditions. Total levels of cellular SGK protein were unaltered 
      upon exposure to dexamethasone, but a marked increase of SGK in a Triton-X100 
      insoluble fraction was observed. BK-channel alpha-subunits could not be 
      co-immunoprecipitated with SGK. In summary, SGK, but not dex-ras1, mRNA is 
      rapidly induced by glucocorticoid stimulation in HEK293 cells. However, there 
      appears to be no direct protein-protein interaction between SGK and BK-channel 
      alpha-subunits.
FAU - Attarzadeh-Yazdi, Ghassem
AU  - Attarzadeh-Yazdi G
AD  - Membrane Biology Group, Centre for Integrative Physiology, University of 
      Edinburgh, Edinburgh, EH8 9XD, UK. gattarzadeh@yahoo.com
FAU - Shipston, Michael J
AU  - Shipston MJ
FAU - Antoni, Ferenc A
AU  - Antoni FA
LA  - eng
GR  - 067683/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
DEP - 20071106
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Potassium Channels, Calcium-Activated)
RN  - 0 (RASD1 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (serum-glucocorticoid regulated kinase)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - Blotting, Northern
MH  - Cell Line, Tumor
MH  - Dexamethasone/*pharmacology
MH  - Genes, Reporter/drug effects/genetics
MH  - Humans
MH  - Immediate-Early Proteins/*genetics
MH  - Immunoprecipitation
MH  - Luciferases/genetics
MH  - Potassium Channels, Calcium-Activated/drug effects
MH  - Protein Serine-Threonine Kinases/*genetics
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - ras Proteins/*genetics
EDAT- 2007/11/07 09:00
MHDA- 2008/06/05 09:00
CRDT- 2007/11/07 09:00
PHST- 2007/09/18 00:00 [accepted]
PHST- 2007/11/07 09:00 [pubmed]
PHST- 2008/06/05 09:00 [medline]
PHST- 2007/11/07 09:00 [entrez]
AID - 10.1007/s11064-007-9516-5 [doi]
PST - ppublish
SO  - Neurochem Res. 2008 Apr;33(4):609-13. doi: 10.1007/s11064-007-9516-5. Epub 2007 
      Nov 6.