PMID- 17986006
OWN - NLM
STAT- MEDLINE
DCOM- 20080115
LR  - 20191210
IS  - 1356-9597 (Print)
IS  - 1356-9597 (Linking)
VI  - 12
IP  - 11
DP  - 2007 Nov
TI  - PER2 controls circadian periods through nuclear localization in the 
      suprachiasmatic nucleus.
PG  - 1225-34
AB  - Molecular circadian clock regulation engages a negative feedback loop comprising 
      components of the negative limb, PERs and CRYs. In addition to the rhythmic 
      transcriptional regulation of clock genes, controlled subcellular localization 
      might contribute to the molecular mechanism of the mammalian circadian clock. To 
      address this issue, we generated transgenic (TG) mice lines harboring either rat 
      PER2 (rPER2) with a deleted nuclear localizing domain [NLD(-)] or intact PER2. In 
      comparison with wild-type (WT) control, the period of the circadian locomotor 
      rhythm in TG mice over-expressing NLD(-) PER2 was longer, while that in TG mice 
      over-expressing intact PER2 was shorter. The nuclear entry of endogenous PER2, 
      CRY1 and CRY2 was delayed in the suprachiasmatic nucleus (SCN) of NLD(-) PER2 TG 
      mice under constant darkness, whereas that of mouse PER2 (mPER2) is accelerated 
      in the SCN of intact PER2 TG mice. Under constant light, the locomotor activity 
      of NLD(-) PER2 TG mice became arrhythmic, whereas WT animals remained rhythmic. 
      These data indicate that PER2 controls circadian periods through nuclear 
      localization in the SCN. In addition, sleep architecture was also affected in 
      intact PER2 TG mice, suggesting PER2 can modulate a sleep molecular mechanism.
FAU - Miyazaki, Koyomi
AU  - Miyazaki K
AD  - Clock Cell Biology Group, Institute for Biological Resource and Function, 
      National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 
      Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
FAU - Wakabayashi, Miyuki
AU  - Wakabayashi M
FAU - Chikahisa, Sachiko
AU  - Chikahisa S
FAU - Sei, Hiroyoshi
AU  - Sei H
FAU - Ishida, Norio
AU  - Ishida N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Genes Cells
JT  - Genes to cells : devoted to molecular & cellular mechanisms
JID - 9607379
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cry1 protein, mouse)
RN  - 0 (Cry1 protein, rat)
RN  - 0 (Cry2 protein, mouse)
RN  - 0 (Cry2 protein, rat)
RN  - 0 (Cryptochromes)
RN  - 0 (Flavoproteins)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Per2 protein, rat)
RN  - 0 (Period Circadian Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Body Temperature
MH  - COS Cells
MH  - Cell Cycle Proteins/genetics/*metabolism
MH  - Cell Nucleus/chemistry/*metabolism
MH  - Chlorocebus aethiops
MH  - *Circadian Rhythm/physiology
MH  - Cryptochromes
MH  - Flavoproteins/analysis/genetics/metabolism
MH  - Mice
MH  - Models, Biological
MH  - Nuclear Localization Signals
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Period Circadian Proteins
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Suprachiasmatic Nucleus/*metabolism
EDAT- 2007/11/08 09:00
MHDA- 2008/01/16 09:00
CRDT- 2007/11/08 09:00
PHST- 2007/11/08 09:00 [pubmed]
PHST- 2008/01/16 09:00 [medline]
PHST- 2007/11/08 09:00 [entrez]
AID - GTC1129 [pii]
AID - 10.1111/j.1365-2443.2007.01129.x [doi]
PST - ppublish
SO  - Genes Cells. 2007 Nov;12(11):1225-34. doi: 10.1111/j.1365-2443.2007.01129.x.