PMID- 17988298
OWN - NLM
STAT- MEDLINE
DCOM- 20071226
LR  - 20211020
IS  - 1751-5521 (Print)
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 29
IP  - 6
DP  - 2007 Dec
TI  - Mean platelet size related to glycoprotein-specific autoantibodies and 
      platelet-associated IgG.
PG  - 433-41
AB  - Recent evidence suggests that platelet-associated glycoprotein-specific (GP) 
      antibodies represent true positive autoantibodies and can therefore be taken as 
      the gold standard. Earlier tests, which aimed at detecting platelet-associated 
      IgG (PA-IgG), might have been hampered, e.g. by the variation of platelet size in 
      thrombocytopenic patients. In this study, 206 samples with increased PA-IgG from 
      consecutive thrombocytopenic patients were tested further for GP-specific 
      antibodies with a monoclonal antibody immobilized platelet antigen test (MAIPA) 
      using a combination of a GP IIbIIIa-specific and a GP IbIX-specific antibody for 
      immobilization or, in a separate assay, GP V-specific antibody. Mean platelet 
      size was recorded as forward scatter (FSC) of platelets in flow cytometric 
      analysis of PA-IgG. GP-specific antibodies were detected in 49 (24%) of the 206 
      patient samples. Their presence correlated well with increased PA-IgG (R = 
      0.769). The mean platelet size and mean fluorescence intensity (MFI) of PA-IgG 
      were both significantly increased in patients compared with healthy controls (n = 
      112; P < 0.0001). Notably, PA-IgG was associated with platelet size within the 
      platelet population of both healthy controls and patients (R = 0.999). Further, 
      the probability of GP IIbIIIa and/or IbIX and GP V-specific PA-IgG tended to 
      increase with the mean platelet size of the patients (P = 0.045). In conclusion, 
      large platelets bound more IgG than platelets of normal size, which may explain 
      at least in part the reported low specificity of total PA-IgG measurement. As the 
      PA-IgG displays low specificity compared with the gold standard, its use as such 
      may be abandoned and replaced by tests for platelet-associated GP-specific 
      autoantibodies.
FAU - Javela, K
AU  - Javela K
AD  - Finnish Red Cross Blood Service, Helsinki, Finland. kaija.javela@bts.redcross.fi
FAU - Kekomaki, R
AU  - Kekomaki R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
RN  - 0 (Autoantibodies)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Platelet Membrane Glycoproteins)
SB  - IM
MH  - Autoantibodies/*blood/immunology
MH  - *Cell Size
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunoassay
MH  - Immunoglobulin G/*blood/immunology
MH  - Male
MH  - Platelet Membrane Glycoproteins/*immunology
MH  - Thrombocytopenia/*blood/immunology
PMC - PMC2228396
EDAT- 2007/11/09 09:00
MHDA- 2007/12/27 09:00
PMCR- 2008/02/05
CRDT- 2007/11/09 09:00
PHST- 2007/11/09 09:00 [pubmed]
PHST- 2007/12/27 09:00 [medline]
PHST- 2007/11/09 09:00 [entrez]
PHST- 2008/02/05 00:00 [pmc-release]
AID - CLH885 [pii]
AID - 10.1111/j.1751-553X.2007.00885.x [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2007 Dec;29(6):433-41. doi: 10.1111/j.1751-553X.2007.00885.x.