PMID- 17989611
OWN - NLM
STAT- MEDLINE
DCOM- 20080103
LR  - 20211203
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 84
IP  - 8
DP  - 2007 Oct 27
TI  - KIR gene and KIR ligand analysis to predict graft rejection after renal 
      transplantation.
PG  - 1045-51
AB  - BACKGROUND: The identification of transplant patients at high risk for rejection 
      after reduction of immunosuppression would allow minimization of 
      immunosuppression and avoidance of side effects in low-risk patients. Next to T 
      cells, innate natural killer (NK) cells may contribute to graft rejection. NK 
      cell activation depends on the balance between activating and inhibitory signals, 
      delivered by self-human leukocyte antigens (HLA) through binding of killer-cell 
      immunoglobulin receptors (KIR). In transplantation, KIR and/or HLA mismatching 
      may lead to NK cell activation. METHODS: In this study, we have evaluated whether 
      acute rejection after reduction of immunosuppression after renal transplantation 
      was associated with peripheral blood NK cell frequencies or with predicted NK 
      cell alloreactivity based on KIR gene and ligand analysis. HLA and KIR genotyping 
      was used to analyze the presence of single KIR genes and haplotypes, and to 
      predict NK cell alloreactivity based on the "missing self" and "missing ligand" 
      hypothesis. NK cell frequencies were analyzed using flow cytometry. RESULTS: No 
      association was found between NK cell alloreactivity based on KIR gene analysis 
      or peripheral blood NK cell subset frequencies and the occurrence of acute 
      rejection after reduction of immunosuppression. CONCLUSIONS: Our data suggest 
      that in a setting where immunosuppression is reduced, prior analysis of NK cell 
      reactivity cannot identify patients at risk for subsequent graft rejection.
FAU - Kreijveld, Ellen
AU  - Kreijveld E
AD  - Department of Blood Transfusion and Transplantation Immunology, Radboud 
      University Nijmegen Medical Centre, Nijmegen, the Netherlands.
FAU - van der Meer, Arnold
AU  - van der Meer A
FAU - Tijssen, Henk J
AU  - Tijssen HJ
FAU - Hilbrands, Luuk B
AU  - Hilbrands LB
FAU - Joosten, Irma
AU  - Joosten I
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
RN  - 0 (Ligands)
RN  - 0 (Receptors, KIR)
SB  - IM
MH  - Adult
MH  - Epitopes/analysis
MH  - Female
MH  - Graft Rejection/*epidemiology/genetics/*prevention & control
MH  - HLA Antigens/analysis
MH  - Haplotypes
MH  - Humans
MH  - *Immunosuppression Therapy
MH  - Incidence
MH  - Kidney/immunology
MH  - *Kidney Transplantation
MH  - Killer Cells, Natural/*immunology
MH  - Ligands
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Receptors, KIR/*genetics
EDAT- 2007/11/09 09:00
MHDA- 2008/01/04 09:00
CRDT- 2007/11/09 09:00
PHST- 2007/11/09 09:00 [pubmed]
PHST- 2008/01/04 09:00 [medline]
PHST- 2007/11/09 09:00 [entrez]
AID - 00007890-200710270-00018 [pii]
AID - 10.1097/01.tp.0000286097.11173.70 [doi]
PST - ppublish
SO  - Transplantation. 2007 Oct 27;84(8):1045-51. doi: 
      10.1097/01.tp.0000286097.11173.70.