PMID- 17989613
OWN - NLM
STAT- MEDLINE
DCOM- 20080103
LR  - 20220227
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 84
IP  - 8
DP  - 2007 Oct 27
TI  - Generation of immunosuppressive mesenchymal stem cells in allogeneic human serum.
PG  - 1055-9
AB  - Mesenchymal stem cells (MSC) may be used to treat acute graft-versus-host disease 
      and for tissue repair. In vitro expansion of MSC has been achieved in the 
      presence of fetal calf serum (FCS). For safety and regulatory reasons, we 
      explored if FCS could be replaced by human blood group AB serum. Proliferation 
      and fold increase of MSC was higher in the presence of AB-serum, compared to FCS. 
      Similar to cells generated in FCS media, MSC from AB-serum media were more than 
      95% positive for CD90, CD105 and human leukocyte antigen (HLA) class I, and 
      negative for hematopoietic and endothelial markers CD14, CD31, CD34, CD45, and 
      CD80. HLA class II expression was higher in MSC generated in AB-serum, but 
      decreased with higher passage numbers. MSC generated in AB-serum suppressed 
      lymphocyte proliferation in mixed lymphocyte cultures and after stimulation with 
      phytohemagglutinin. MSC expanded in AB-serum and FCS have similar in vitro 
      properties.
FAU - Le Blanc, Katarina
AU  - Le Blanc K
AD  - Division of Clinical Immunology, Karolinska Institutet, Karolinska University 
      Hospital Huddinge, Stockholm, Sweden. Katarina.Leblanc@ki.se
FAU - Samuelsson, Hakan
AU  - Samuelsson H
FAU - Lonnies, Lena
AU  - Lonnies L
FAU - Sundin, Mikael
AU  - Sundin M
FAU - Ringden, Olle
AU  - Ringden O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (ABO Blood-Group System)
RN  - 0 (Antigens, CD)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - ABO Blood-Group System
MH  - Adolescent
MH  - Adult
MH  - Animals
MH  - Antigens, CD/analysis
MH  - Cattle
MH  - *Cell Culture Techniques
MH  - Cell Proliferation
MH  - Child
MH  - Female
MH  - Graft vs Host Disease/*therapy
MH  - Histocompatibility Antigens Class I/analysis
MH  - Histocompatibility Antigens Class II/analysis
MH  - Humans
MH  - Immunosuppression Therapy/*methods
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/*immunology
MH  - Serum/immunology
MH  - Transplantation, Homologous
EDAT- 2007/11/09 09:00
MHDA- 2008/01/04 09:00
CRDT- 2007/11/09 09:00
PHST- 2007/11/09 09:00 [pubmed]
PHST- 2008/01/04 09:00 [medline]
PHST- 2007/11/09 09:00 [entrez]
AID - 00007890-200710270-00020 [pii]
AID - 10.1097/01.tp.0000285088.44901.ea [doi]
PST - ppublish
SO  - Transplantation. 2007 Oct 27;84(8):1055-9. doi: 
      10.1097/01.tp.0000285088.44901.ea.