PMID- 17991550
OWN - NLM
STAT- MEDLINE
DCOM- 20071214
LR  - 20220317
IS  - 1527-9995 (Electronic)
IS  - 0090-4295 (Linking)
VI  - 70
IP  - 4
DP  - 2007 Oct
TI  - Fluorescence in situ hybridization for detecting upper urinary tract tumors--a 
      preliminary report.
PG  - 753-7
AB  - OBJECTIVES: To evaluate fluorescence in situ hybridization (FISH) in voided urine 
      specimens and selective upper urinary tract washings obtained from patients with 
      suspected transitional cell cancer of the upper urinary tract (UT-TCC). METHODS: 
      A total of 16 patients with suspected UT-TCC were included in the study. All 
      patients underwent urinary tract imaging. Endoscopy was also performed. Voided 
      urine specimens from all patients and ureteral washings were subjected to 
      cytologic examination and FISH analysis (UroVysion probe set). If indicated, 
      surgery (nephroureterectomy or ureterectomy) was performed. RESULTS: Of the 16 
      patients, 9 (56.25%) were diagnosed with UT-TCC. None of the remaining patients 
      was diagnosed with UT-TCC after a mean follow-up of 21.3 months (median 20, range 
      11 to 36). For FISH analysis, the sensitivity was 87.5% and specificity 80%. FISH 
      analysis was not possible in 1 patient with UT-TCC because of an insufficient 
      number of cells. For cytology, the sensitivity was 60% and specificity 80%. In 
      patients with UT-TCC, the cytologic findings were inconclusive (atypia of 
      uncertain significance) in 4 patients (44.4%). In contrast to the cytology 
      findings, all results of the FISH analysis of the upper urinary tract washings 
      matched those of the voided urine samples. CONCLUSIONS: Our preliminary data 
      suggest that FISH analysis of voided urine might be a useful ancillary test in 
      the detection of UT-TCC with excellent sensitivity and specificity. If confirmed 
      in larger studies, FISH might contribute to a more reliable and less-invasive 
      diagnostic approach to UT-TCC.
FAU - Akkad, Thomas
AU  - Akkad T
AD  - Department of Urology, Medical University Innsbruck, Innsbruck, Austria.
FAU - Brunner, Andrea
AU  - Brunner A
FAU - Pallwein, Leo
AU  - Pallwein L
FAU - Gozzi, Christian
AU  - Gozzi C
FAU - Bartsch, Georg
AU  - Bartsch G
FAU - Mikuz, Gregor
AU  - Mikuz G
FAU - Steiner, Hannes
AU  - Steiner H
FAU - Verdorfer, Irmgard
AU  - Verdorfer I
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Urology
JT  - Urology
JID - 0366151
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Transitional Cell/*diagnosis/surgery/urine
MH  - Cytodiagnosis
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Kidney Neoplasms/*diagnosis/surgery/urine
MH  - Male
MH  - Middle Aged
MH  - Predictive Value of Tests
MH  - Sensitivity and Specificity
MH  - Ureteral Neoplasms/*diagnosis/surgery/urine
MH  - Urine/cytology
EDAT- 2007/11/10 09:00
MHDA- 2007/12/15 09:00
CRDT- 2007/11/10 09:00
PHST- 2006/05/04 00:00 [received]
PHST- 2007/04/23 00:00 [revised]
PHST- 2007/06/26 00:00 [accepted]
PHST- 2007/11/10 09:00 [pubmed]
PHST- 2007/12/15 09:00 [medline]
PHST- 2007/11/10 09:00 [entrez]
AID - S0090-4295(07)01782-7 [pii]
AID - 10.1016/j.urology.2007.06.1103 [doi]
PST - ppublish
SO  - Urology. 2007 Oct;70(4):753-7. doi: 10.1016/j.urology.2007.06.1103.