PMID- 17993667
OWN - NLM
STAT- MEDLINE
DCOM- 20080321
LR  - 20071112
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 395
DP  - 2007
TI  - WebACT: an online genome comparison suite.
PG  - 57-74
AB  - Comparison of related genomes is an enormously powerful technique for explaining 
      phenotypic differences and revealing recent evolutionary events. Genomes evolve 
      through a host of mechanisms including long- and short-range intragenomic 
      rearrangements, insertion of laterally acquired DNA, gene loss, and 
      single-nucleotide polymorphisms. The Artemis Comparison Tool (ACT) was developed 
      to enable the intuitive visualization of the consequences of such events in the 
      context of two or more aligned genomes. WebACT is an online resource designed to 
      allow the alignment of up to five genomic sequences within the ACT environment 
      without the need for local software installation. Comparisons can be carried out 
      between uploaded sequences, or those selected from the EMBL or RefSeq databases, 
      using BLASTZ, MUMmer, or Basic Local Alignment Search Tool (BLAST). Precomputed 
      comparisons can be selected from a database covering all the completed bacterial 
      chromosome and plasmid sequences in the Genome Reviews database (1). This allows 
      the rapid visualization of regions of interest, without the need to handle the 
      full genome sequences. Here, we describe the process of using WebACT to prepare 
      comparisons for visualization, and the selection of precomputed comparisons from 
      the database. The use of ACT to view the selected comparison is then explored 
      using examples from bacterial genomes.
FAU - Abbott, James C
AU  - Abbott JC
AD  - Centre for Bioinformatics, Imperial College London, UK.
FAU - Aanensen, David M
AU  - Aanensen DM
FAU - Bentley, Stephen D
AU  - Bentley SD
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - *Databases, Genetic
MH  - *Genome
MH  - *Internet
MH  - Polymorphism, Single Nucleotide
MH  - Sequence Alignment
MH  - User-Computer Interface
EDAT- 2007/11/13 09:00
MHDA- 2008/03/22 09:00
CRDT- 2007/11/13 09:00
PHST- 2007/11/13 09:00 [pubmed]
PHST- 2008/03/22 09:00 [medline]
PHST- 2007/11/13 09:00 [entrez]
AID - 1-59745-514-8:57 [pii]
PST - ppublish
SO  - Methods Mol Biol. 2007;395:57-74.